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Metabotropic Glutamate Receptor 1 antibody (Glutamate Receptor, Metabotropic 1) (APC)

Details for Product anti-GRM1 Antibody No. ABIN807922, Supplier: Log in to see
Antigen
  • fj39d05
  • wu:fj39d05
  • si:ch211-51a19.1
  • Glura
  • mGlutR
  • GB18621
  • GPRC1A
  • MGLU1
  • MGLUR1
  • SCAR13
  • 4930455H15Rik
  • ENSMUSG00000075319
  • Gm10828
  • Gprc1a
  • mGluR1
  • nmf373
  • rcw
  • wobl
  • glutamate receptor, metabotropic 1
  • glutamate receptor, metabotropic 1a
  • metabotropic glutamate receptor 1
  • GRM1
  • grm1a
  • mGlutR1
  • Grm1
Reactivity
Human, Non-Human Primate
118
47
44
6
6
3
3
2
1
1
1
1
1
1
Host
Mouse
112
16
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Metabotropic Glutamate Receptor 1 antibody is conjugated to APC
5
5
5
4
4
4
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
86
54
52
32
13
10
6
3
3
2
2
2
2
1
1
Options
Supplier
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Immunogen Human neutrophils
Clone 3G8
Isotype IgG1
Specificity The mouse monoclonal antibody 3G8 recognizes CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA, 50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils).

HLDA V, WS Code NK80
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Alternative Name GRM1 (GRM1 Antibody Abstract)
Background CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms –, FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Gene ID 2214
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Product cited in: Burt, Plitas, Zhao, Bamboat, Nguyen, Dupont, DeMatteo: "The lytic potential of human liver NK cells is restricted by their limited expression of inhibitory killer Ig-like receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 3, pp. 1789-96, 2009 (PubMed).

Choi, Wang, Peterson, Letvin, Reimann: "Use of an anti-CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques." in: Immunology, Vol. 124, Issue 2, pp. 215-22, 2008 (PubMed).

Congy-Jolivet, Bolzec, Ternant, Ohresser, Watier, Thibault: "Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa-158V allotype." in: Cancer research, Vol. 68, Issue 4, pp. 976-80, 2008 (PubMed).

Komano, Nanki, Hayashida, Taniguchi, Miyasaka: "Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts." in: Arthritis research & therapy, Vol. 8, Issue 5, pp. R152, 2007 (PubMed).

Wijngaarden, van Roon, van de Winkel, Bijlsma, Lafeber: "Down-regulation of activating Fcgamma receptors on monocytes of patients with rheumatoid arthritis upon methotrexate treatment." in: Rheumatology (Oxford, England), Vol. 44, Issue 6, pp. 729-34, 2005 (PubMed).

Metes, Ernst, Chambers, Sulica, Herberman, Morel: "Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene." in: Blood, Vol. 91, Issue 7, pp. 2369-80, 1998 (PubMed).

Background publications Zhu, Hamann, Muñoz, Rubio, Mayer, Hernrreiter, Leff: "Intracellular expression of Fc gamma RIII (CD16) and its mobilization by chemoattractants in human eosinophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 5, pp. 2574-9, 1998 (PubMed).