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Notch1 antibody (Notch 1) (Intracellular)

Details for Product anti-NOTCH1 Antibody No. ABIN807923, Supplier: Log in to see
Antigen
  • notch1
  • NOTCH1
  • hn1
  • tan1
  • notch
  • xotch
  • xnotch
  • notch-1
  • xnotch1
  • TAN1
  • hN1
  • 9930111A19Rik
  • Mis6
  • N1
  • Tan1
  • lin-12
  • NOTCH
  • Xotch
  • notch1-a
  • notch 1
  • Notch homolog 1, translocation-associated (Drosophila)
  • Notch homolog 1, translocation-associated
  • notch1
  • NOTCH1
  • Notch1
Alternatives
anti-Human Notch1 antibody for Western Blotting
Epitope
Intracellular
54
48
36
23
17
17
13
13
9
7
7
6
5
5
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
268
198
67
6
2
2
2
2
2
2
1
Host
Mouse
172
169
14
8
6
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Notch1 antibody is un-conjugated
25
15
13
9
7
6
5
5
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
223
126
108
83
76
67
61
24
15
11
5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
Options
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Immunogen GST fusion protein containing cdc10-NCR region of mouse Notch1
Clone MN1A
Isotype IgG1
Specificity The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.
No Cross-Reactivity Rat (Rattus)
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name NOTCH1 (NOTCH1 Antibody Abstract)
Background Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged1 (CD339), and Jagged2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.
Gene ID 4851
Pathways Notch Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Khwaja, Liu, Tong, Jin, Pear, van Deursen, Bram: "HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice." in: The Journal of clinical investigation, Vol. 120, Issue 7, pp. 2537-48, 2010 (PubMed).

Watanabe, Nagaoka, Lee, Bianco, Gonzales, Castro, Rangel, Sakamoto, Sun, Callahan, Salomon: "Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1." in: The Journal of cell biology, Vol. 187, Issue 3, pp. 343-53, 2009 (PubMed).

Sun, Li, Vercherat, Gulbagci, Acharjee, Li, Chung, Thin, Taneja: "Stra13 regulates satellite cell activation by antagonizing Notch signaling." in: The Journal of cell biology, Vol. 177, Issue 4, pp. 647-57, 2007 (PubMed).

Huppert, Le, Schroeter, Mumm, Saxena, Milner, Kopan: "Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1." in: Nature, Vol. 405, Issue 6789, pp. 966-70, 2000 (PubMed).

Background publications Kefas, Comeau, Floyd, Seleverstov, Godlewski, Schmittgen, Jiang, diPierro, Li, Chiocca, Lee, Fine, Abounader, Lawler, Purow: "The neuronal microRNA miR-326 acts in a feedback loop with notch and has therapeutic potential against brain tumors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 48, pp. 15161-8, 2009 (PubMed).

Tagami, Okochi, Yanagida, Ikuta, Fukumori, Matsumoto, Ishizuka-Katsura, Nakayama, Itoh, Jiang, Nishitomi, Kamino, Morihara, Hashimoto, Tanaka, Kudo, Chiba, Takeda: "Regulation of Notch signaling by dynamic changes in the precision of S3 cleavage of Notch-1." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 165-76, 2007 (PubMed).

Espinosa, Santos, Inglés-Esteve, Muñoz-Canoves, Bigas: "p65-NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N-CoR." in: Journal of cell science, Vol. 115, Issue Pt 6, pp. 1295-303, 2002 (PubMed). Further details: �