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Notch1 antibody (Notch 1) (Intracellular)

Details for Product anti-NOTCH1 Antibody No. ABIN807923, Supplier: Log in to see
Antigen
  • notch1
  • NOTCH1
  • hn1
  • tan1
  • notch
  • xotch
  • xnotch
  • notch-1
  • xnotch1
  • TAN1
  • hN1
  • 9930111A19Rik
  • Mis6
  • N1
  • Tan1
  • lin-12
  • NOTCH
  • Xotch
  • notch1-a
  • notch 1
  • Notch homolog 1, translocation-associated (Drosophila)
  • Notch homolog 1, translocation-associated
  • notch1
  • NOTCH1
  • Notch1
Alternatives
anti-Human Notch1 antibody for Western Blotting
Epitope
Intracellular
48
37
34
23
15
13
8
6
6
6
5
5
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
281
219
101
7
5
5
4
2
2
2
2
1
1
1
1
Host
Mouse
181
169
13
8
6
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Notch1 antibody is un-conjugated
25
15
13
9
9
7
6
6
6
5
5
5
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
221
136
105
82
74
68
65
24
16
13
6
5
4
3
3
2
2
2
2
2
2
2
1
1
1
1
Options
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Immunogen GST fusion protein containing cdc10-NCR region of mouse Notch1
Clone MN1A
Isotype IgG1
Specificity The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.
No Cross-Reactivity Rat (Rattus)
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name NOTCH1 (NOTCH1 Antibody Abstract)
Background Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged1 (CD339), and Jagged2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.
Gene ID 4851
Pathways Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Khwaja, Liu, Tong, Jin, Pear, van Deursen, Bram: "HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice." in: The Journal of clinical investigation, Vol. 120, Issue 7, pp. 2537-48, 2010 (PubMed).

Watanabe, Nagaoka, Lee, Bianco, Gonzales, Castro, Rangel, Sakamoto, Sun, Callahan, Salomon: "Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1." in: The Journal of cell biology, Vol. 187, Issue 3, pp. 343-53, 2009 (PubMed).

Sun, Li, Vercherat, Gulbagci, Acharjee, Li, Chung, Thin, Taneja: "Stra13 regulates satellite cell activation by antagonizing Notch signaling." in: The Journal of cell biology, Vol. 177, Issue 4, pp. 647-57, 2007 (PubMed).

Huppert, Le, Schroeter, Mumm, Saxena, Milner, Kopan: "Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1." in: Nature, Vol. 405, Issue 6789, pp. 966-70, 2000 (PubMed).

Background publications Kefas, Comeau, Floyd, Seleverstov, Godlewski, Schmittgen, Jiang, diPierro, Li, Chiocca, Lee, Fine, Abounader, Lawler, Purow: "The neuronal microRNA miR-326 acts in a feedback loop with notch and has therapeutic potential against brain tumors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 48, pp. 15161-8, 2009 (PubMed).

Tagami, Okochi, Yanagida, Ikuta, Fukumori, Matsumoto, Ishizuka-Katsura, Nakayama, Itoh, Jiang, Nishitomi, Kamino, Morihara, Hashimoto, Tanaka, Kudo, Chiba, Takeda: "Regulation of Notch signaling by dynamic changes in the precision of S3 cleavage of Notch-1." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 165-76, 2007 (PubMed).

Espinosa, Santos, Inglés-Esteve, Muñoz-Canoves, Bigas: "p65-NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N-CoR." in: Journal of cell science, Vol. 115, Issue Pt 6, pp. 1295-303, 2002 (PubMed). Further details: �