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Notch1 antibody

NOTCH1 Reactivity: Human, Mouse WB, FACS, ICC, IP, IHC (fro) Host: Mouse Monoclonal mN1A unconjugated
Catalog No. ABIN807923
  • Target See all Notch1 (NOTCH1) Antibodies
    Notch1 (NOTCH1) (Notch 1 (NOTCH1))
    Reactivity
    • 114
    • 88
    • 49
    • 9
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 94
    • 38
    • 6
    • 3
    • 2
    • 1
    Mouse
    Clonality
    • 89
    • 51
    • 2
    Monoclonal
    Conjugate
    • 66
    • 10
    • 9
    • 8
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Notch1 antibody is un-conjugated
    Application
    • 94
    • 50
    • 47
    • 30
    • 27
    • 26
    • 22
    • 20
    • 14
    • 11
    • 9
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.
    No Cross-Reactivity
    Rat
    Cross-Reactivity (Details)
    Human, Mouse
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    GST fusion protein containing cdc10-NCR region of mouse Notch1
    Clone
    MN1A
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product NOTCH1 Primary Antibody
  • Application Notes
    Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Khwaja, Liu, Tong, Jin, Pear, van Deursen, Bram: "HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice." in: The Journal of clinical investigation, Vol. 120, Issue 7, pp. 2537-48, (2010) (PubMed).

    Watanabe, Nagaoka, Lee, Bianco, Gonzales, Castro, Rangel, Sakamoto, Sun, Callahan, Salomon: "Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1." in: The Journal of cell biology, Vol. 187, Issue 3, pp. 343-53, (2009) (PubMed).

    Sun, Li, Vercherat, Gulbagci, Acharjee, Li, Chung, Thin, Taneja: "Stra13 regulates satellite cell activation by antagonizing Notch signaling." in: The Journal of cell biology, Vol. 177, Issue 4, pp. 647-57, (2007) (PubMed).

    Huppert, Le, Schroeter, Mumm, Saxena, Milner, Kopan: "Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1." in: Nature, Vol. 405, Issue 6789, pp. 966-70, (2000) (PubMed).

  • Target
    Notch1 (NOTCH1) (Notch 1 (NOTCH1))
    Alternative Name
    Notch 1 (NOTCH1 Products)
    Synonyms
    notch1 antibody, NOTCH1 antibody, hn1 antibody, tan1 antibody, notch antibody, xotch antibody, xnotch antibody, notch-1 antibody, xnotch1 antibody, TAN1 antibody, hN1 antibody, 9930111A19Rik antibody, Mis6 antibody, N1 antibody, Tan1 antibody, lin-12 antibody, NOTCH antibody, Xotch antibody, notch1-a antibody, notch 1 antibody, fibropellin-1 antibody, Notch homolog 1, translocation-associated (Drosophila) antibody, Notch homolog 1, translocation-associated antibody, notch 1 S homeolog antibody, notch1 antibody, LOC100631526 antibody, NOTCH1 antibody, Notch1 antibody, notch1.S antibody
    Background
    Notch receptor 1,Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged 1 (CD339), and Jagged 2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.,AOS5, TAN1, hN1, AOVD1
    Gene ID
    4851
    UniProt
    P46531
    Pathways
    Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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