Endoglin (ENG) antibody (Biotin)

Details for Product No. ABIN810077, Supplier: Log in to see
Antigen
  • ENG
  • MGC137842
  • DKFZp469D0419
  • END
  • HHT1
  • ORW1
  • AI528660
  • AI662476
  • CD105
  • S-endoglin
  • endoglin
  • ENG
  • LOC100226246
  • Eng
Reactivity
Human, Pig (Porcine)
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3
1
1
1
1
Host
Mouse
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228
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5
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
Biotin
66
63
49
21
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2
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1
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
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Options
Supplier
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Supplier Product No.
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Immunogen Recombinant Vaccinia virus containing the human CD105 (L-isoform) cDNA.
Clone MEM-229
Isotype IgG2a
Specificity The antibody MEM-229 recognizes CD105 (Endoglin), a 90 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow, it is also present on syncytiotrophoblast on placenta throughout pregnancy.
No Cross-Reactivity Dog (Canine), Horse (Equine)
Characteristics The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Alternative Name CD105 (ENG Antibody Abstract)
Background CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.
Gene ID 2022
Research Area Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Immunofluorescence (IF) image for anti-Endoglin (ENG) antibody (Biotin) (ABIN810077) Immunofluorescence staining of an infarcted porcine heart with anti-CD105 (MEM-229 ;
Product cited in: Plánka, Necas, Srnec, Rauser, Starý, Jancár, Amler, Filová, Hlucilová, Kren, Gál: "Use of allogenic stem cells for the prevention of bone bridge formation in miniature pigs." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 58, Issue 6, pp. 885-93, 2010 (PubMed).

Background publications Piao, Tokunaga: "Significant expression of endoglin (CD105), TGFbeta-1 and TGFbeta R-2 in the atherosclerotic aorta: an immunohistological study." in: Journal of atherosclerosis and thrombosis, Vol. 13, Issue 2, pp. 82-9, 2006 (PubMed).

Warrington, Hillarby, Li, Letarte, Kumar: "Functional role of CD105 in TGF-beta1 signalling in murine and human endothelial cells." in: Anticancer research, Vol. 25, Issue 3B, pp. 1851-64, 2005 (PubMed).

Guo, Slevin, Li, Parameshwar, Liu, Kumar, Bernabeu, Kumar: "CD105 inhibits transforming growth factor-beta-Smad3 signalling." in: Anticancer research, Vol. 24, Issue 3a, pp. 1337-45, 2004 (PubMed).

Zhu, Sun, Xie, Jin, Sheibani, Greenberg: "Hypoxic induction of endoglin via mitogen-activated protein kinases in mouse brain microvascular endothelial cells." in: Stroke; a journal of cerebral circulation, Vol. 34, Issue 10, pp. 2483-8, 2003 (PubMed).

Li, Issa, Kumar, Hampson, Lopez-Novoa, Bernabeu, Kumar: "CD105 prevents apoptosis in hypoxic endothelial cells." in: Journal of cell science, Vol. 116, Issue Pt 13, pp. 2677-85, 2003 (PubMed).

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