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Caspase 3 ELISA Kit

CASP3 Reactivity: Rat Colorimetric Sandwich ELISA 0.312-20 ng/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN811984
  • Target See all Caspase 3 (CASP3) ELISA Kits
    Caspase 3 (CASP3)
    Reactivity
    • 9
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.312-20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Application
    ELISA
    Purpose
    For the quantitative determination of rat caspase 3 (Casp-3) concentrations in serum, plasma, tissue homogenates.
    Sample Type
    Serum, Plasma, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of rat Casp-3.
    Cross-Reactivity (Details)
    Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.
    Sensitivity
    0.078 ng/mL
    Components
    • Assay plate (12 × 8 coated Microwells)
    • Standard (freeze dried)
    • Biotin-antibody (100 × concentrate)
    • HRP-avidin (100 × concentrate)
    • Biotin-antibody Diluent
    • HRP-avidin Diluent
    • Sample Diluent
    • Wash Buffer (25 × concentrate)
    • TMB Substrate
    • Stop Solution
    • Adhesive Strip (for 96 wells)
    • Instruction manual
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  • Application Notes
    • The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
    • Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
    • Grossly hemolyzed samples are not suitable for use in this assay.
    • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
    • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
    • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
    • Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
    • Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
    • Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
    Comment

    Detection wavelength: 450 nm

    Information on standard material:
    Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

    Information on reagents:
    In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

    Information on antibodies:
    The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

    Sample Volume
    100 μL
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Casp-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Casp-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Casp-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Casp-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Reagent Preparation
    • Biotin-antibody (1×) - Centrifuge the vial before opening.
      Biotin-antibody requires a 100-fold dilution. The suggested dilution is 10µL of Biotin-antibody + 990µL of Biotin-antibody Diluent.
    • HRP-avidin (1×) - Centrifuge the vial before opening.
      HRP-avidin requires a 100-fold dilution. The suggested dilution is 10µL of HRP-avidin + 990µL of HRP-avidin Diluent.
    • Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20mL of Wash Buffer Concentrate (25×) into deionized or distilled water to prepare 500mL of Wash Buffer (1×).
    • Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
      Reconstitute the Standard with 1ml of Sample Diluent. Do not substitute other diluents. This reconstitution produces a stock solution. Mix the standard to ensure complete reconstitution and allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making dilutions.
      Pipette 250µL of Sample Diluent into each tube. Use the stock solution to produce a 2-fold dilution series. Mix each tube thoroughly before the next transfer. The undiluted Standard serves as the high standard. Sample Diluent serves as the zero standard (0ng/mL).
    Note:
    • Kindly use graduated containers to prepare the reagent. Please don't prepare the reagent directly in the Diluent vials provided in the kit.
    • Bring all reagents to room temperature (18-25°C) before use for 30 min.
    • Prepare fresh standard for each assay. Use within 4 hours and discard after use.
    • Making serial dilution in the wells directly is not permitted.
    • Please carefully reconstitute Standards according to the instruction. Avoid foaming and mix gently until the crystals have completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10µL when pipetting.
    • It is recommended to use distilled water to prepare reagents and samples. Using contaminated water or container for reagent preparation will influence detection result.
    Assay Precision
    Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
    Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.
    • Intra-assay: CV% less than 8%
    • Inter-assay: CV% less than 10%
    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.
    Handling Advice
    • The kit should not be used beyond the expiration date on the kit label.
    • Do not mix or substitute reagents with those from other lots or sources.
    • If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
    • Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
    • This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    Storage
    4 °C/-20 °C
    Storage Comment
    For unopened kit: All the reagents should be kept according to the labels on vials.
    Expiry Date
    6 months
  • Salama, Tadros, Schaalan, Bahaa, Abdel-Tawab, Khalifa: "Potential neuroprotective effect of androst-5-ene-3β, 17β-diol (ADIOL) on the striatum, and substantia nigra in Parkinson's disease rat model." in: Journal of cellular physiology, Vol. 233, Issue 8, pp. 5981-6000, (2019) (PubMed).

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    Zhao, Tang, Zhang, Liu, Li: "Magnesium isoglycyrrhizinate protects against renal‑ischemia‑reperfusion injury in a rat model via anti‑inflammation, anti‑oxidation and anti‑apoptosis." in: Molecular medicine reports, Vol. 16, Issue 3, pp. 3627-3633, (2018) (PubMed).

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    El-Shemi, Kensara, Alsaegh, Mukhtar et al.: "Pharmacotherapy with Thymoquinone Improved Pancreatic β-Cell Integrity and Functional Activity, Enhanced Islets Revascularization, and Alleviated Metabolic and Hepato-Renal Disturbances in ..." in: Pharmacology, Vol. 101, Issue 1-2, pp. 9-21, (2018) (PubMed).

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    Alawdi, El-Denshary, Safar, Eidi, David, Abdel-Wahhab: "Neuroprotective Effect of Nanodiamond in Alzheimer's Disease Rat Model: a Pivotal Role for Modulating NF-κB and STAT3 Signaling." in: Molecular neurobiology, (2016) (PubMed).

    Em, Ar, Am, Aa: "Thymol and Carvacrol Prevent Cisplatin-Induced Nephrotoxicity by Abrogation of Oxidative Stress, Inflammation, and Apoptosis in Rats." in: Journal of biochemical and molecular toxicology, (2015) (PubMed).

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    Mahmoud-Awny, Attia, Abd-Ellah, El-Abhar: "Mangiferin Mitigates Gastric Ulcer in Ischemia/ Reperfused Rats: Involvement of PPAR-?, NF-?B and Nrf2/HO-1 Signaling Pathways." in: PLoS ONE, Vol. 10, Issue 7, pp. e0132497, (2015) (PubMed).

    El-Sayed, Mansour, Abdul-Hameed: "Thymol and Carvacrol Prevent Doxorubicin-Induced Cardiotoxicity by Abrogation of Oxidative Stress, Inflammation, and Apoptosis in Rats." in: Journal of biochemical and molecular toxicology, (2015) (PubMed).

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    El-Ansary, Al-Daihan, El-Gezeery: "On the protective effect of omega-3 against propionic acid-induced neurotoxicity in rat pups." in: Lipids in health and disease, Vol. 10, pp. 142, (2011) (PubMed).

  • Target See all Caspase 3 (CASP3) ELISA Kits
    Caspase 3 (CASP3)
    Alternative Name
    Caspase 3, Apoptosis-Related Cysteine Peptidase (CASP3) (CASP3 Products)
    Synonyms
    CPP32 ELISA Kit, CPP32B ELISA Kit, SCA-1 ELISA Kit, A830040C14Rik ELISA Kit, AC-3 ELISA Kit, Apopain ELISA Kit, CC3 ELISA Kit, Caspase-3 ELISA Kit, Lice ELISA Kit, Yama ELISA Kit, mldy ELISA Kit, xcpp32 ELISA Kit, casp3 ELISA Kit, zgc:100890 ELISA Kit, CASP-3 ELISA Kit, caspase-3 ELISA Kit, caspase 3 ELISA Kit, caspase 3 S homeolog ELISA Kit, caspase 3, apoptosis-related cysteine peptidase a ELISA Kit, caspase 3, apoptosis-related cysteine peptidase ELISA Kit, CASP3 ELISA Kit, Casp3 ELISA Kit, casp3.S ELISA Kit, casp3a ELISA Kit
    Background
    Synonyms: CPP32, CPP32B, SCA-1, PARP cleavage protease|SREBP cleavage activity 1|Yama|apopain|caspase 3|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3
    HGNC
    1504
    UniProt
    Q8MJC3
    Pathways
    Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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