DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 1-387) antibody

Details for Product No. ABIN863134
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Synonyms DNMT3L, D6Ertd14e, ecat7
AA 1-387
Human, Mouse (Murine)
Clonality (Clone)
Monoclonal ()
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
Pubmed 8 references available
Quantity 100 μg
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Availability Will be delivered in 3 to 4 Business Days
Immunogen Fusion protein amino acids 1-387 of human DNMT3L
Clone S117-9
Specificity Detects ~45 kDa. No cross-reactivity against DNMT3a or DNMT3B.
Sensitivity 1 µg/mL of SMC-341 was sufficient for detection of DNMT3L in 10 µg of COS cell transiently transfected with Flag-tagged DNMT3L lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Purification Protein G Purified
Background Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue (1-6). Studies show that DNMT3L regulates the activity of DNMT3A and DNMT3B and stimulates their catalytic activities (7). DNMT3L has specifically been linked to the process of carcinogenesis, thru its role in nuclear programming (8).
Cellular Localization: Nucleus
Gene ID 29947
NCBI Accession NP_037501
UniProt Q9UJW3
Research Area Chromatin and Nuclear Signaling, Epigenetics
Application Notes Recommended Dilution: WB (1:1000), IHC (1:1000), ICC/IF (1:100)
Optimal dilutions for assays should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1) (AA 1-387) antibody (ABIN863134) SMC 341DNMT3LRat Tissue Mix Western Blotting.
Background publications Gokul, Ramakrishna, Khosla: "Reprogramming of HeLa cells upon DNMT3L overexpression mimics carcinogenesis." in: Epigenetics : official journal of the DNA Methylation Society, Vol. 4, Issue 5, pp. 322-9, 2009 (PubMed).

Kareta, Botello, Ennis et al.: "Reconstitution and mechanism of the stimulation of de novo methylation by human DNMT3L." in: The Journal of biological chemistry, Vol. 281, Issue 36, pp. 25893-902, 2006 (PubMed).

Robertson, Ait-Si-Ali, Yokochi et al.: "DNMT1 forms a complex with Rb, E2F1 and HDAC1 and represses transcription from E2F-responsive promoters." in: Nature genetics, Vol. 25, Issue 3, pp. 338-42, 2000 (PubMed).

Reik, Kelsey, Walter: "Dissecting de novo methylation." in: Nature genetics, Vol. 23, Issue 4, pp. 380-2, 1999 (PubMed).

Okano, Bell, Haber et al.: "DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development." in: Cell, Vol. 99, Issue 3, pp. 247-57, 1999 (PubMed).

Xie, Wang, Okano et al.: "Cloning, expression and chromosome locations of the human DNMT3 gene family." in: Gene, Vol. 236, Issue 1, pp. 87-95, 1999 (PubMed).

Bestor, Laudano, Mattaliano et al.: "Cloning and sequencing of a cDNA encoding DNA methyltransferase of mouse cells. The carboxyl-terminal domain of the mammalian enzymes is related to bacterial restriction methyltransferases." in: Journal of molecular biology, Vol. 203, Issue 4, pp. 971-83, 1989 (PubMed).

Yen, Vertino, Nelkin et al.: "Isolation and characterization of the cDNA encoding human DNA methyltransferase." in: Nucleic acids research, Vol. 20, Issue 9, pp. 2287-91, 1992 (PubMed).

Catalog No. ABIN863134
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