Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3) (AA 74-95), (N-Term) antibody

Details for Product No. ABIN863201, Supplier: Log in to see
Antigen
  • SLC12A3
  • slc12a3
  • DKFZp469N2315
  • NCC
  • NCCT
  • TSC
  • AI035291
  • solute carrier family 12 (sodium/chloride transporters), member 3
  • thiazide-sensitive sodium chloride cotransporter
  • solute carrier family 12 (sodium/chloride transporters), member 3, gene 2
  • solute carrier family 12 (sodium/chloride transporter), member 3
  • solute carrier family 12, member 3
  • SLC12A3
  • NCCT
  • slc12a3.2
  • Slc12a3
Epitope
AA 74-95, N-Term
23
19
15
10
3
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
80
40
36
19
3
2
2
2
1
1
1
1
1
Host
Rabbit
79
5
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoelectron Microscopy (IEM), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
70
39
27
23
17
16
13
9
3
3
2
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen Poduced against a synthetic peptide mapping to a segment of rat NCC (amino acids 74-95), N-terminal
Specificity Detects ~160 kDa.
Purification Protein A Purified
Alternative Name NCC (SLC12A3 Antibody Abstract)
Background NCC, a thiazide-sensitive NaCl co-transporter, is found on the apical membrane of the distal convoluted tubule, where it is the principal mediator of Na+ and Cl- reabsorption in this segment of the nephron. It is activated by phosphorylation, and has been implicated in renal NaCl and K+ homeostasis (1). Regulation of NCC expression and phosphorylation by dietary NACl restriction appears to involve SGK1(1). In experiments with angiotensin II-infused mice, increased sensitivity to Ang II may involved over-activity of NCC (2). Therefore, NCC is the target of thiazide diuretics used in the treatment of hypertension (1). Molecular experiments have also shown that NCC has been detected in the lens cortex, core and fiber cells of a rat (3).
Gene ID 54300, 6559
NCBI Accession NP_062218, NP_000330
UniProt P55018, P55017
Application Notes
  • WB (1:1000)
  • IHC (1:200)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SPC-402 was sufficient for detection of NCC3 in 10 μg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
Western Blotting (WB) image for anti-Solute Carrier Family 12 (Sodium/Chloride Transporters), Member 3 (SLC12A3) (AA 74-95), (N-Term) antibody (ABIN863201) NCC Western Blotting, rat tissue.
Product cited in: Gois, Canale, Luchi, Volpini, Veras, Costa, Shimizu, Seguro: "Tenofovir during pregnancy in rats: a novel pathway for programmed hypertension in the offspring." in: The Journal of antimicrobial chemotherapy, Vol. 70, Issue 4, pp. 1094-105, 2015 (PubMed).

Wen, Yuan, Warner, Wang, Cornelius, Wang-France, Li, Boettger, Sansom: "Increased Epithelial Sodium Channel Activity Contributes to Hypertension Caused by Na+-HCO3- Cotransporter Electrogenic 2 Deficiency." in: Hypertension, Vol. 66, Issue 1, pp. 68-74, 2015 (PubMed).

Cheng, Wu, Kortenoeven, Pisitkun, Fenton: "A Systems Level Analysis of Vasopressin-mediated Signaling Networks in Kidney Distal Convoluted Tubule Cells." in: Scientific reports, Vol. 5, pp. 12829, 2015 (PubMed).

Rosenbaek, Kortenoeven, Aroankins, Fenton: "Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis." in: The Journal of biological chemistry, Vol. 289, Issue 19, pp. 13347-61, 2014 (PubMed).

Esteva-Font, Guillén-Gómez, Diaz, Guirado, Facundo, Ars, Ballarin, Fernández-Llama: "Renal sodium transporters are increased in urinary exosomes of cyclosporine-treated kidney transplant patients." in: American journal of nephrology, Vol. 39, Issue 6, pp. 528-35, 2014 (PubMed).

Donnelly, Needham, Snyder, Roy, Khadem, Brodsky, Subramanya: "Hsp70 and Hsp90 multichaperone complexes sequentially regulate thiazide-sensitive cotransporter endoplasmic reticulum-associated degradation and biogenesis." in: The Journal of biological chemistry, Vol. 288, Issue 18, pp. 13124-35, 2013 (PubMed).

Rosenbaek, Assentoft, Pedersen, MacAulay, Fenton: "Characterization of a novel phosphorylation site in the sodium-chloride cotransporter, NCC." in: The Journal of physiology, Vol. 590, Issue Pt 23, pp. 6121-39, 2012 (PubMed).

Background publications Chee, Vorontsova, Lim, Kistler, Donaldson: "Expression of the sodium potassium chloride cotransporter (NKCC1) and sodium chloride cotransporter (NCC) and their effects on rat lens transparency." in: Molecular vision, Vol. 16, pp. 800-12, 2010 (PubMed).

Tiwari, Li, Riazi, Halagappa, Ecelbarger: "Sex and age result in differential regulation of the renal thiazide-sensitive NaCl cotransporter and the epithelial sodium channel in angiotensin II-infused mice." in: American journal of nephrology, Vol. 30, Issue 6, pp. 554-62, 2009 (PubMed).

Vallon, Schroth, Lang, Kuhl, Uchida: "Expression and phosphorylation of the Na+-Cl- cotransporter NCC in vivo is regulated by dietary salt, potassium, and SGK1." in: American journal of physiology. Renal physiology, Vol. 297, Issue 3, pp. F704-12, 2009 (PubMed).

Did you look for something else?