Caspase 3 antibody (Caspase 3, Apoptosis-Related Cysteine Peptidase) (AA 29-33)

Details for Product anti-CASP3 Antibody No. ABIN871475, Supplier: Log in to see
Antigen
  • CC3
  • CG14902
  • Cas3
  • Casp 3
  • Casp3
  • Caspase-3
  • DECAY
  • Decay
  • Dmel\\CG14902
  • Drosophila executioner caspase related to Apopain/Yama
  • caspase 3
  • caspase-3
  • CG7788
  • DRICE
  • Dmel\\CG7788
  • DrICE
  • DrIce
  • Drice
  • ICE
  • crice
  • drICE
  • drIce
  • drice
  • ice
  • A830040C14Rik
  • AC-3
  • Apopain
  • CPP32
  • Lice
  • Yama
  • mldy
  • CPP32B
  • SCA-1
  • xcpp32
  • caspase 3, apoptosis-related cysteine peptidase
  • death executioner caspase related to Apopain/Yama
  • CG7788 gene product from transcript CG7788-RA
  • caspase 3
  • CASP3
  • decay
  • Ice
  • Casp3
  • casp3
Alternatives
anti-Human Caspase 3 antibody for BioImaging
Epitope
AA 29-33
41
32
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24
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16
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Reactivity
Human
567
305
254
48
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38
29
28
24
20
18
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12
9
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2
2
Host
Rabbit
458
162
19
4
2
Clonality
Polyclonal
Conjugate
This Caspase 3 antibody is un-conjugated
27
24
19
14
9
9
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4
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3
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Application
Western Blotting (WB)
483
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219
147
95
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44
29
28
13
9
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2
2
2
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2
1
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Options
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Immunogen Peptide sequence around AA 29-33 (S-G-I-S-L) derived from Caspase 3. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Specificity The antibody detects endogenous level of total Caspase 3 protein.
Purification Affinity purified
Alternative Name Caspase 3 (CASP3 Antibody Abstract)
Background Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
Molecular Weight 17 kDa
Gene ID 836
NCBI Accession NP_004337
UniProt P42574
Research Area Proteolysis / Ubiquitin, Apoptosis/Necrosis
Pathways Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
Application Notes Western blotting: 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Shen, Song, An, Li, Wu, Ruan, Hu, Hu: "The use of hollow mesoporous silica nanospheres to encapsulate bortezomib and improve efficacy for non-small cell lung cancer therapy." in: Biomaterials, Vol. 35, Issue 1, pp. 316-26, 2013 (PubMed).

Background publications Gervais, Xu, Robertson, Vaillancourt, Zhu, Huang, LeBlanc, Smith, Rigby, Shearman, Clarke, Zheng, Van Der Ploeg, Ruffolo, Thornberry, Xanthoudakis, Zamboni, Roy, Nicholson: "Involvement of caspases in proteolytic cleavage of Alzheimer's amyloid-beta precursor protein and amyloidogenic A beta peptide formation." in: Cell, Vol. 97, Issue 3, pp. 395-406, 1999 (PubMed).

Nicholson, Ali, Thornberry, Vaillancourt, Ding, Gallant, Gareau, Griffin, Labelle, Lazebnik: "Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis." in: Nature, Vol. 376, Issue 6535, pp. 37-43, 1995 (PubMed).