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CDK4 antibody (Cyclin-Dependent Kinase 4) (AA 295-299)

Details for Product anti-CDK4 Antibody No. ABIN871488, Supplier: Login to see
Antigen
Alternatives
anti-Human CDK4 antibody for Immunohistochemistry (Frozen Sections)
Epitope
AA 295-299
67
16
15
14
13
10
9
9
8
5
5
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
245
93
68
9
5
4
2
1
Host
Rabbit
166
100
7
3
2
1
Clonality
Polyclonal
Conjugate
This CDK4 antibody is un-conjugated
12
11
9
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
234
98
64
51
49
21
18
16
11
10
5
3
2
2
2
2
2
1
1
1
Supplier
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Immunogen Peptide sequence around AA 295-299 (L-H-K-D-E) derived from Human CDK4. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Specificity The antibody detects endogenous levels of total CDK4 protein.
Purification Affinity purified
Alternative Name CDK4 (CDK4 Antibody Abstract)
Background Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle .
Molecular Weight 34 kDa
Gene ID 1019
NCBI Accession NP_000066
UniProt P11802
Research Area Cell Cycle
Pathways Cell Division Cycle
Application Notes Western blotting: 1:500-1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Tabor: "Prions of mammals and fungi minireview series." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 1, 1999 (PubMed).

Lukas, Bartkova, Bartek: "Convergence of mitogenic signalling cascades from diverse classes of receptors at the cyclin D-cyclin-dependent kinase-pRb-controlled G1 checkpoint." in: Molecular and cellular biology, Vol. 16, Issue 12, pp. 6917-25, 1997 (PubMed).

Sherr: "Cancer cell cycles." in: Science (New York, N.Y.), Vol. 274, Issue 5293, pp. 1672-7, 1997 (PubMed).

Hirai, Roussel, Kato et al.: "Novel INK4 proteins, p19 and p18, are specific inhibitors of the cyclin D-dependent kinases CDK4 and CDK6." in: Molecular and cellular biology, Vol. 15, Issue 5, pp. 2672-81, 1995 (PubMed).