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MAP2K4 antibody (Mitogen-Activated Protein Kinase Kinase 4) (AA 352-356)

Details for Product anti-MAP2K4 Antibody No. ABIN871490, Supplier: Log in to see
Antigen
  • 2
  • 142758_at
  • ARABIDOPSIS THALIANA MITOGEN-ACTIVATED PROTEIN KINASE KINASE 4
  • ATMEK4
  • ATMKK4
  • CG9738
  • CT27508
  • CT38386
  • D-MKK4
  • DmelCG12798
  • Dmel\\CG9738
  • dMkk4
  • DMKK4
  • dMKK4
  • F19C24.26
  • F19C24_26
  • hep
  • jnkk
  • JNKK
  • JNKK1
  • jnkk1
  • Jnkk2
  • JNKK2
  • map2k4
  • MAP KINASE KINASE 4
  • MAPKK4
  • mapkk4
  • Mapkk7
  • MEK4
  • mek4
  • Mek7
  • mitogen-activated protein kinase kinase 4
  • MKK4
  • mkk4
  • MKK4A-l
  • Mkk7
  • Mpk4
  • PRKMK4
  • prkmk4
  • SAPKK-1
  • SAPKK1
  • Sek1
  • sek1
  • SEK1
  • SEK1/MKK4
  • serk1
  • SERK1
  • Serk1
  • SKK1
  • xmek2
  • zgc:136500
Alternatives
anti-Human MAP2K4 antibody for Western Blotting
Epitope
AA 352-356
56
42
28
24
22
19
15
13
11
5
5
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
305
146
115
9
8
5
4
2
Host
Rabbit
281
37
Clonality
Polyclonal
Conjugate
This MAP2K4 antibody is un-conjugated
12
12
11
8
8
8
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
206
149
117
62
41
18
14
13
10
10
4
3
1
Supplier
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Immunogen Peptide sequence around AA 352-356 (E-S-K-R-P) derived from Human MAP2K4. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Specificity The antibody detects endogenous levels of total MAP2K4 protein.
Purification Affinity purified
Alternative Name MAP2K4 (MAP2K4 Antibody Abstract)
Background SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflamatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved T*PY* site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction
Molecular Weight 44 kDa
Gene ID 6416
NCBI Accession NP_003001
UniProt P45985
Pathways MAPK Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway
Application Notes Western blotting: 1:500-1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Park, Kim, Huh, Park, Chung, Kang, Choi: "Akt (protein kinase B) negatively regulates SEK1 by means of protein phosphorylation." in: The Journal of biological chemistry, Vol. 277, Issue 4, pp. 2573-8, 2002

Davis: "MAPKs: new JNK expands the group." in: Trends in biochemical sciences, Vol. 19, Issue 11, pp. 470-3, 1995

Sánchez, Hughes, Mayer, Yee, Woodgett, Avruch, Kyriakis, Zon: "Role of SAPK/ERK kinase-1 in the stress-activated pathway regulating transcription factor c-Jun." in: Nature, Vol. 372, Issue 6508, pp. 794-8, 1995

Lehrmann: "US to fund project to develop desk-top DNA screening tool." in: Nature, Vol. 372, Issue 6501, pp. 8, 1994

Kyriakis, Banerjee, Nikolakaki, Dai, Rubie, Ahmad, Avruch, Woodgett: "The stress-activated protein kinase subfamily of c-Jun kinases." in: Nature, Vol. 369, Issue 6476, pp. 156-60, 1994