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Synaptophysin 2 (AA 257-261) antibody

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Antigen
Epitope
AA 257-261
2
1
Reactivity
Rat (Rattus)
4
1
Host
Rabbit
4
Clonality
Polyclonal
Application
Western Blotting (WB)
4
Supplier
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Immunogen Peptide sequence around AA 257-261 (G-P-T-S-F) derived from Rat synaptophysin 2. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Specificity The antibody detects endogenous levels of total synaptophysin 2 protein.
Purification Affinity purified
Background Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms, and thus can be used as a marker in tumor diagnosis (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex
Molecular Weight 37 kDa
Gene ID 66030
NCBI Accession NP_076464
UniProt P22831
Application Notes Western blotting: 1:500-1:1000
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Bonanomi, Rusconi, Colombo et al.: "Synaptophysin I selectively specifies the exocytic pathway of synaptobrevin 2/VAMP2." in: The Biochemical journal, Vol. 404, Issue 3, pp. 525-34, 2007 (PubMed).

Baker, Ju, Chiu: "Identification of secondary structure elements in intermediate-resolution density maps." in: Structure (London, England : 1993), Vol. 15, Issue 1, pp. 7-19, 2007 (PubMed).

Valtorta, Pennuto, Bonanomi et al.: "Synaptophysin: leading actor or walk-on role in synaptic vesicle exocytosis?" in: BioEssays : news and reviews in molecular, cellular and developmental biology, Vol. 26, Issue 4, pp. 445-53, 2004 (PubMed).

Wiedenmann, Franke, Kuhn et al.: "Synaptophysin: a marker protein for neuroendocrine cells and neoplasms." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 10, pp. 3500-4, 1986 (PubMed).