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GFAP antibody (Glial Fibrillary Acidic Protein) (AA 423-427)

Details for Product anti-GFAP Antibody No. ABIN871512, Supplier: Login to see
Antigen
Epitope
AA 423-427
71
70
30
28
23
13
13
6
6
6
5
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
579
215
205
97
95
39
26
24
19
16
11
10
6
5
4
4
3
3
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
353
304
29
25
4
3
2
Clonality
Polyclonal
Conjugate
This GFAP antibody is un-conjugated
29
23
19
11
11
11
7
4
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
1
1
1
1
Application
Western Blotting (WB)
508
298
185
183
169
165
77
54
33
21
14
12
11
10
9
8
7
6
5
3
1
1
1
1
1
1
1
1
1
Supplier
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Immunogen Peptide sequence around AA 423-427 (E-S-K-Q-E) derived from Human GFAP. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
Specificity The antibody detects endogenous level of total GFAP protein.
Purification Affinity purified
Alternative Name GFAP (GFAP Antibody Abstract)
Background GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Molecular Weight 50 kDa
Gene ID 2670
NCBI Accession NP_002046
UniProt P14136
Research Area Stem Cells, Cytoskeleton, Glia marker, Cell/Tissue Markers, Neurology
Pathways
Application Notes Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Background publications Nielsen, Holm, Johansen et al.: "A new splice variant of glial fibrillary acidic protein, GFAP epsilon, interacts with the presenilin proteins." in: The Journal of biological chemistry, Vol. 277, Issue 33, pp. 29983-91, 2002 (PubMed).

Bongcam-Rudloff, Nistér, Betsholtz et al.: "Human glial fibrillary acidic protein: complementary DNA cloning, chromosome localization, and messenger RNA expression in human glioma cell lines of various phenotypes." in: Cancer research, Vol. 51, Issue 5, pp. 1553-60, 1991 (PubMed).