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CDK4 antibody (AA 295-299)

CDK4 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN871659
  • Target See all CDK4 Antibodies
    CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
    Binding Specificity
    • 23
    • 16
    • 15
    • 9
    • 9
    • 9
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 295-299
    Reactivity
    • 117
    • 73
    • 53
    • 19
    • 6
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 108
    • 31
    • 2
    • 1
    Rabbit
    Clonality
    • 107
    • 34
    Polyclonal
    Conjugate
    • 81
    • 11
    • 11
    • 6
    • 6
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CDK4 antibody is un-conjugated
    Application
    • 119
    • 55
    • 37
    • 31
    • 18
    • 18
    • 16
    • 15
    • 14
    • 13
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Specificity
    The antibody detects endogenous levels of total CDK4 protein.
    Purification
    Affinity purified
    Immunogen
    Peptide sequence around AA 295-299 (L-H-K-D-E) derived from Human CDK4. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.
    Top Product
    Discover our top product CDK4 Primary Antibody
  • Application Notes
    Western blotting: 1:500-1:1000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Target
    CDK4 (Cyclin-Dependent Kinase 4 (CDK4))
    Alternative Name
    CDK4 (CDK4 Products)
    Synonyms
    zgc:153726 antibody, CMM3 antibody, PSK-J3 antibody, Crk3 antibody, 8-6 antibody, CDK4 antibody, CDK4/6 antibody, CG5072 antibody, Cdk4/6 antibody, DmCdk4 antibody, Dmel\\CG5072 antibody, Pk53C antibody, Pk?7 antibody, cdk antibody, cdk4 antibody, cdk4/6 antibody, dCdk4 antibody, l(2)05428 antibody, l(2)0671 antibody, l(2)k06503 antibody, l(2)s4639 antibody, l(2)sh0671 antibody, xcdk4 antibody, cyclin-dependent kinase 4 antibody, cyclin dependent kinase 4 antibody, Cyclin-dependent kinase 4 antibody, cyclin-dependent kinase 4 L homeolog antibody, cdk4 antibody, CDK4 antibody, Cdk4 antibody, cdk4.L antibody
    Background
    Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins . The inactive ternary complex of CDK4/cyclin D and p27 Kip1/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase . The active complex of CDK4/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression . In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4/cyclin D3 leads to a G2 delay, implicating CDK4/cyclin D3 activity in progression through the G2-phase of the cell cycle .
    Molecular Weight
    34 kDa
    Gene ID
    1019
    NCBI Accession
    NP_000066
    UniProt
    P11802
    Pathways
    Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size
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