Synaptophysin 2 antibody (AA 257-261)

Catalog No. ABIN871665

Product Details anti-Synaptophysin 2 Antibody

Target: Synaptophysin 2

Binding Specificity: AA 257-261

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Synaptophysin 2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody detects endogenous levels of total synaptophysin 2 protein.

Purification: Affinity purified

Immunogen: Peptide sequence around AA 257-261 (G-P-T-S-F) derived from Rat synaptophysin 2. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for Synaptophysin 2

Target: Synaptophysin 2

Abstract: Synaptophysin 2 Products

Synonyms: synaptoporin antibody, Synpr antibody

Background: Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms, and thus can be used as a marker in tumor diagnosis (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex

Molecular Weight: 37 kDa

Gene ID: 66030

NCBI Accession: NP_076464

UniProt: P22831