CDC42 antibody (pSer71) (Alexa Fluor 647)

Catalog No. ABIN876858

Product Details anti-CDC42 Antibody (Alexa Fluor 647)

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Binding Specificity: pSer71

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC42 antibody is conjugated to Alexa Fluor 647

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human CDC42 around the phosphorylation site of Ser71

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CDC42

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Alternative Name: CDC42 (CDC42 Products)

Synonyms: CDC42Hs antibody, G25K antibody, AI747189 antibody, AU018915 antibody, cdc42a antibody, wu:fb07d08 antibody, wu:fj62g06 antibody, zgc:63918 antibody, Xcdc42 antibody, cdc42hs antibody, g25k antibody, CDC42 antibody, CG12530 antibody, Cdc42Dm antibody, D-CDC42 antibody, D-Cdc42 antibody, D-cdc42 antibody, DCDC42 antibody, DCdc42 antibody, Dcdc42 antibody, Dm Cdc42 antibody, DmCDC42 antibody, Dmcdc42 antibody, Dmel\\CG12530 antibody, cdc42 antibody, cell division cycle 42 antibody, Cell division control protein 42 homolog antibody, cell division control protein 42 homolog antibody, cell division cycle 42 L homeolog antibody, CG12530 gene product from transcript CG12530-RC antibody, Rho family GTPase antibody, CDC42 antibody, Cdc42 antibody, cdc42 antibody, LOC100636735 antibody, LOC100637699 antibody, LOC100638834 antibody, cdc42.L antibody

Background:

Synonyms: CDC 42, CDC42Hs, Cell division control protein 42 homolog, Cell division cycle 42, Cell division cycle 42 isoform 1, Cell division cycle 42 isoform 2, dJ224A6.1.1, dJ224A6.1.2, G25K, G25K GTP binding protein, Growth regulating protein, GTP binding protein 25kD, Small GTP binding protein CDC42.

Background: The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013].

Gene ID: 998

Pathways: MAPK Signaling, Microtubule Dynamics, RTK Signaling, WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2, EGFR Downregulation, VEGF Signaling