CDC14B antibody (AA 65-160) (Alexa Fluor 350)

Catalog No. ABIN889444

Product Details anti-CDC14B Antibody (Alexa Fluor 350)

Target: CDC14B (CDC14 Cell Division Cycle 14B (CDC14B))

Binding Specificity: AA 65-160

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC14B antibody is conjugated to Alexa Fluor 350

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CDC14B

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for CDC14B

Target: CDC14B (CDC14 Cell Division Cycle 14B (CDC14B))

Alternative Name: CDC14B (CDC14B Products)

Background:

Synonyms: CDC14B3, Cdc14B1, Cdc14B2, hCDC14B, Dual specificity protein phosphatase CDC14B, CDC14 cell division cycle 14 homolog B, CDC14B

Background: Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylates SIRT2 around early anaphase. Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases.

Gene ID: 8555

UniProt: O60729