HBSAg antibody (Alexa Fluor 350)

Catalog No. ABIN900187

Product Details anti-HBSAg Antibody (Alexa Fluor 350)

Target: HBSAg (HBsAg) (Hepatitis B Virus Surface Antigen (HBsAg))

Reactivity: Virus, Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This HBSAg antibody is conjugated to Alexa Fluor 350

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Virus

Predicted Reactivity: Bee

Purification: Purified by Protein A.

Immunogen: Human Hepatitis B Surface Antigen

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HBSAg

Target: HBSAg (HBsAg) (Hepatitis B Virus Surface Antigen (HBsAg))

Alternative Name: Hbsag (HBsAg Products)

Target Type: Viral Protein

Background:

Synonyms: HBsAg, HBV major surface antigen, HBV surface antigen, Hepatitis B Virus major surface antigen, Major surface antigen, S, Human Hepatitis B Surface Antigen

Background: Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.

UniProt: Q67926