P4HTM antibody (AA 201-300) (AbBy Fluor® 647)

Catalog No. ABIN900726

The AbBy Fluor® 647-conjugated Rabbit Polyclonal anti-P4HTM antibody (ABIN900726) specifically detects P4HTM in WB, IF (cc) and IF (p). The antibody is reactive with Human samples.

Quick Overview for P4HTM antibody (AA 201-300) (AbBy Fluor® 647) (ABIN900726)

Target: P4HTM (Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This P4HTM antibody is conjugated to AbBy Fluor® 647

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-P4HTM Antibody (AbBy Fluor® 647)

Binding Specificity: AA 201-300

Predicted Reactivity: Human,Mouse,Rat,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HIFPH4

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for P4HTM

Target: P4HTM (Prolyl 4-Hydroxylase, Transmembrane (Endoplasmic Reticulum) (P4HTM))

Alternative Name: HPH4

Background:

Synonyms: EGLN4, H prolyl hydroxylase PH4, H-PH4, H-prolyl hydroxylase 4, HPH4, HPH-4, Hypoxia inducible factor prolyl 4 hydroxylase, Hypoxia inducible factor prolyl hydroxylase, Hypoxia-inducible factor prolyl hydroxylase 4, P4H TM, P4H with transmembrane domain, P4H-TM, P4htm, P4HTM_HUMAN, PH 4, PH4, PHD4, Proline 4 hydroxylase, Prolyl 4 hydroxylase transmembrane endoplasmic reticulum, Prolyl hydroxlase domain containing 4, Transmembrane prolyl 4-hydroxylase.

Background: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.