Human Papilloma Virus 33 E6 (HPV-33 E6) (AA 51-149) antibody (Cy7)

Catalog No. ABIN901228

Product Details

Target: Human Papilloma Virus 33 E6 (HPV-33 E6)

Binding Specificity: AA 51-149

Reactivity: Alphapapillomavirus, Human Papillomavirus (HPV), Virus

Host: Rabbit

Clonality: Polyclonal

Conjugate: Cy7

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Specificity: This antibody will detect E6 in HPV33, HPV58, and Alphapapillomavirus 9.

Cross-Reactivity: Virus

Cross-Reactivity (Details): HPV33, HPV58, Alphapapillomavirus 9.

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HPV33 E6 protein

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Human Papilloma Virus 33 E6 (HPV-33 E6)

Alternative Name: HPV33 E6

Target Type: Viral Protein

Background:

Synonyms: E6 protein [Human papillomavirus type 33], Human Papilloma Virus, Human papillomavirus type 33, Human papillomavirus type 33, Protein 33, HPV33-E6 protein, HPV33 E6 protein, VE6_HPV33.

Background: Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90 % of cervical cancers contain HPV DNA of the high risk types. Mutational analysis have shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro. Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. The E6 protein is a transcriptional transactivator. Binds double-stranded DNA. This protein may be involved in the oncogenic potential of this virus (cervical neoplasia-associated virus).