RNF144B antibody (AA 218-303) (HRP)

Catalog No. ABIN901623

Product Details anti-RNF144B Antibody (HRP)

Target: RNF144B (Ring Finger Protein 144B (RNF144B))

Binding Specificity: AA 218-303

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF144B antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Predicted Reactivity: Human,Mouse,Rat,Dog,Cow,Sheep,Pig,Horse,Chicken,Cat,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human IBRDC2/RING finger protein 144B

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for RNF144B

Target: RNF144B (Ring Finger Protein 144B (RNF144B))

Alternative Name: RNF144B (RNF144B Products)

Synonyms: IBRDC2 antibody, PIR2 antibody, bA528A10.3 antibody, p53RFP antibody, BC025007 antibody, E130105P19Rik antibody, Ibrdc2 antibody, Pir2 antibody, ring finger protein 144B antibody, RNF144B antibody, Rnf144b antibody

Background:

Synonyms: E3 ubiquitin protein ligase RNF144B, RING finger protein 144B, E3 ubiquitin-protein ligase RNF144B, IBR domain containing 2, IBR domain containing protein 2, IBR domain-containing protein 2, IBRDC 2, IBRDC2, p53 inducible RING finger protein, p53-inducible RING finger protein, p53RFP, PIR2, R144B_HUMAN, Ring finger 144B, RING finger protein 144B, RNF 144B, RNF144B.

Background: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death, But, it is not an anti-apoptotic protein per se.Tissue specificity: Broadly expressed, with lowest levels in brain and thymus, and highest levels detectable in heart, ovary and testis.

Gene ID: 255488