Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

RAG1 antibody (AA 351-450) (Cy7)

RAG1 Reactivity: Mouse WB, FACS, IF (p), IF (cc) Host: Rabbit Polyclonal Cy7
Catalog No. ABIN912869
  • Target See all RAG1 Antibodies
    RAG1 (Recombination Activating Gene 1 (RAG1))
    Binding Specificity
    • 15
    • 2
    • 1
    • 1
    • 1
    AA 351-450
    Reactivity
    • 18
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 20
    • 1
    Rabbit
    Clonality
    • 20
    • 1
    Polyclonal
    Conjugate
    • 7
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RAG1 antibody is conjugated to Cy7
    Application
    • 21
    • 13
    • 12
    • 12
    • 4
    • 3
    • 3
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))
    Cross-Reactivity
    Mouse
    Predicted Reactivity
    Human,Rat,Sheep,Pig
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human RAG1/RNF74
    Isotype
    IgG
    Top Product
    Discover our top product RAG1 Primary Antibody
  • Application Notes
    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    RAG1 (Recombination Activating Gene 1 (RAG1))
    Alternative Name
    RNF74 (RAG1 Products)
    Synonyms
    RAG-1 antibody, rag1 antibody, RNF74 antibody, Rag-1 antibody, rag-1 antibody, rag1a antibody, recombination activating 1 antibody, recombination activating gene 1 antibody, recombination activating gene 1 S homeolog antibody, Rag1 antibody, RAG1 antibody, rag1 antibody, rag1.S antibody
    Background

    Synonyms: RAG-1, RNF74, V(D)J recombination-activating protein 1, RING finger protein 74, RAG1

    Background: Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T-lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.

    Gene ID
    5896
    UniProt
    P15918
You are here:
Support