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SMURF2 antibody (AA 601-700) (Alexa Fluor 647)

SMURF2 Reactivity: Human, Mouse, Rat WB, FACS, IF (cc), IF (p) Host: Rabbit Polyclonal Alexa Fluor 647
Catalog No. ABIN915887
  • Target See all SMURF2 Antibodies
    SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))
    Binding Specificity
    • 15
    • 9
    • 8
    • 7
    • 7
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 601-700
    Reactivity
    Human, Mouse, Rat
    Host
    • 63
    • 3
    Rabbit
    Clonality
    • 55
    • 11
    Polyclonal
    Conjugate
    • 21
    • 7
    • 7
    • 6
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SMURF2 antibody is conjugated to Alexa Fluor 647
    Application
    • 54
    • 34
    • 29
    • 18
    • 13
    • 13
    • 12
    • 12
    • 7
    • 3
    • 2
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    Cross-Reactivity
    Human, Mouse, Rat
    Predicted Reactivity
    Cow,Horse,Chicken
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic peptide derived from human SMURF2
    Isotype
    IgG
    Top Product
    Discover our top product SMURF2 Primary Antibody
  • Application Notes
    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    SMURF2 (SMAD Specific E3 Ubiquitin Protein Ligase 2 (SMURF2))
    Alternative Name
    SMURF2 (SMURF2 Products)
    Synonyms
    SMURF2 antibody, 2810411E22Rik antibody, AI558114 antibody, AI649275 antibody, RGD1310067 antibody, wu:fb82a11 antibody, wu:fc41c02 antibody, SMAD specific E3 ubiquitin protein ligase 2 antibody, SMAD specific E3 ubiquitin protein ligase 2 S homeolog antibody, SMURF2 antibody, smurf2 antibody, Smurf2 antibody, smurf2.S antibody
    Background

    Synonyms: E3 ubiquitin-protein ligase SMURF2, hSMURF2, SMAD ubiquitination regulatory factor 2, SMAD-specific E3 ubiquitin-protein ligase 2, SMURF2

    Background: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.

    Gene ID
    64750
    UniProt
    Q9HAU4
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