ZW10 antibody (Alexa Fluor 350)

Catalog No. ABIN920491

Product Details anti-ZW10 Antibody (Alexa Fluor 350)

Target: ZW10 (Zw10 Kinetochore Protein (ZW10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZW10 antibody is conjugated to Alexa Fluor 350

Application: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Predicted Reactivity: Human,Mouse,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse ZW10

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ZW10

Target: ZW10 (Zw10 Kinetochore Protein (ZW10))

Abstract: ZW10 Products

Synonyms: HZW10 antibody, KNTC1AP antibody, 6330566F14Rik antibody, MmZw10 antibody, zw10 kinetochore protein antibody, ZW10 antibody, Zw10 antibody

Background:

Synonyms: Centromere/kinetochore protein zw10, Centromere/kinetochore protein zw10 homolog, HZW 10, HZW10, Kinetochore associated homolog, KNTC1AP, MGC149821, Zeste White 10, Zeste white 10 homolog, ZW 10, ZW 10, centromere/kinetochore protein, ZW 10 kinetochore associated homolog, ZW10 Drosophila homolog centromere/kinetochore protein, ZW10 homolog centromere/kinetochore protein Drosophila, ZW10 homolog centromere/kinetochore protein, ZW10 kinetochore associated homolog, ZW10_MOUSE.

Background: The mitotic checkpoint ensures that chromosomes are divided equally between daughter cells and is a primary mechanism preventing the chromosome instability often seen in aneuploid human tumors. This gene encodes a protein that is one of many involved in mechanisms to ensure proper chromosome segregation during cell division. The encoded protein binds to centromeres during the prophase, metaphase, and early anaphase cell division stages and to kinetochore microtubules during metaphase. It is part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. In mitotic human cells ZW10 resides in a complex with Rod and Zwilch, whereas another ZW10 partner, Zwint-1, is part of a separate complex of structural kinetochore components including Mis12 and Ndc80-Hec1. Zwint-1 is critical for recruiting ZW10 to unattached kinetochores. Depletion from human cells demonstrates that the ZW10 complex is essential for stable binding of a Mad1-Mad2 complex to unattached kinetochores. Thus, ZW10 functions as a linker between the core structural elements of the outer kinetochore and components that catalyze generation of the mitotic checkpoint-derived "stop anaphase" inhibitor.

Gene ID: 9183