His Tag antibody
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- Target See all His Tag Antibodies
- His Tag
- Reactivity
- Please inquire
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This His Tag antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Sequence
- HHHHHH
- Purification
- Affinity purified
- Top Product
- Discover our top product His Tag Primary Antibody
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- Application Notes
- ELISA: Use at a 1:200 - 1:500 dilution for coating. Use at a 1:2,000 - 1:30,000 dilution for detection. Western blot: Use at a 1:1,000 - 1:3,000 dilution.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 1.0 mL of affinity-purified polyclonal antibody in PBS, pH 7.2 and 0.1% sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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- Target
- His Tag
- Alternative Name
- Hexahistidine-Tag (His Tag Products)
- Target Type
- Tag
- Background
- Rabbits were immunized with HHHHHH peptide conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity-purified using the peptide immobilized on a solid support.
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