Myc Tag antibody

Details for Product No. ABIN933570, Supplier: Log in to see
Antigen
Reactivity
Tag
212
36
14
10
6
5
3
1
1
Host
Mouse
160
60
17
16
3
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
27
23
14
9
5
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
215
115
77
53
48
34
22
19
19
7
6
5
5
5
4
4
3
3
3
2
1
1
1
1
1
1
1
Options
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Available images

Immunogen Myc Tag antibody was raised in mouse using EQKLISEEDL (Myc) synthetic peptide conjugated to KLH as the immunogen.
Sequence EQKLISEEDL
Clone Myc-A7
Isotype IgG1
Purification Protein A affinity chromatography
Target Type Tag
Background A myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein using recombinant DNA technology. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A myc tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a myc-tag allows one to follow the protein with an antibody against the Myc epitope. Examples are cellular localization studies by immunofluorescence or detection by SDS-PAGE protein electrophoresis. Synonyms: Monoclonal Myc Tag antibody, anti-Myc Tag antibody, Myc antibody, C-myc antibody, Cmyc antibody.
Research Area Tags/Labels
Application Notes WB: 1:1000-3000
Optimal conditions should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3
Preservative Sodium azide
Precaution of Use This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
Storage RT/-20 °C
Storage Comment Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.
Supplier Images
Western Blotting (WB) image for anti-Myc Tag antibody (ABIN933570) 1:1000 (1 ug/ml) antibody dilution probed against HEK 293 cells transfected with Myc-...