Lhcii Type III Chlorophyll A/B-Binding Protein (Lhcb3) antibody

Antigen: Lhcii Type III Chlorophyll A/B-Binding Protein (Lhcb3)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Peanut (Arachis hypogaea), Barley (Hordeum vulgare), Tomato (Solanum lycopersicum), Mesembryanthemum crystallinum, Tobacco (Nicotiana tabacum), Rice (Oryza sativa), Pea (Pisum sativum), Spinach (Spinacia oleracea), Maize/Corn (Zea mays)

Application: Western Blotting (WB)

Catalog no.: ABIN93508

Additional Information

Alternative name: LHCII type III chlorophyll a/b-binding protein (Lhcb3 )

Immunogen: BSA-conjugated synthetic peptide derived from a highly conserved sequence ofLhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms,including Arabidopsis thaliana (At5g54270). This sequence is highly conservedeven in Ginko biloba and one of the major LHCII-forms of Physcomitrella patens

Format: Lyophilized

Description: The major light-harvesting antenna complex II (LHCII) in photsynthetic eukaryotesis located in the thylakoid membrane of the chloroplast. It is a heterotrimericcomplex formed by up to 3 different individual subtypes of chlorophyll a/b-bindingproteins: Lhcb1, Lhcb2, and Lhcb3. While Lhcb1 and Lhcb2 are quite similar andregularily present in multiple gene-copies, the Lhcb3 protein differs inpigment-composition and molecular size and often is coded by only a single gene.Lhcb3 seems not to be present in the mobile LHCII trimers involved in state1-state 2 transitions.

Specificity: Predicted reactivity: angiosperms (monocots and dicots), gymnosperms, mosses.

Application Details

Application Notes: Recommended Dilution 1 : 2000 with standard ECL (WB). Additional Information: A molecular characterisation of the LHCII proteins can be found in Caffarri et al.(2004) A Look within LHCII: Differential Analysis of the Lhcb1−3 ComplexesBuilding the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis.Biochemistry 43 (29): 9467–9476 .

Buffer: Total IgG in PBS pH 7.4, lyophilized

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Publications

Publications: Andersson, Walters, Horton et al.: "Antisense inhibition of the photosynthetic antenna proteins CP29 and CP26: implications for the mechanism of protective energy dissipation." in: The Plant cell, Vol. 13, Issue 5, pp. 1193-204, 2001 (PubMed).

Tikkanen, Nurmi, Suorsa et al.: "Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants." in: Biochimica et biophysica acta, Vol. 1777, Issue 5, pp. 425-32, 2008 (PubMed).

Jung, Lee, Lee et al.: "Toxic tetrapyrrole accumulation in protoporphyrinogen IX oxidase-overexpressing transgenic rice plants." in: Plant molecular biology, Vol. 67, Issue 5, pp. 535-46, 2008 (PubMed).