Lhcii Type II Chlorophyll A/B-Binding Protein (Lhcb2) antibody

Antigen: Lhcii Type II Chlorophyll A/B-Binding Protein (Lhcb2)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Peanut (Arachis hypogaea), Barley (Hordeum vulgare), Tomato (Solanum lycopersicum), Mesembryanthemum crystallinum, Tobacco (Nicotiana tabacum), Rice (Oryza sativa), Pea (Pisum sativum), Spinach (Spinacia oleracea), Maize/Corn (Zea mays), Chlamydomonas reinhardtii (C. reinhardtii)

Application: Western Blotting (WB)

Catalog no.: ABIN93509

Additional Information

Immunogen: BSA-conjugated synthetic peptide derived from a highly conserved sequence ofLhb1 proteins from angiosperms (monocots and dicots) and gymnosperms,including Arabidopsis thaliana At2g05100 (b2.1), At2g05070 (b2.2), At3g27690(b2.4) and also in a 4th isoform (b2.3, protein accession AF134124) listed byJansson (1999) that is not annotated in the current TAIR database [2009-03-02].

Format: Lyophilized

Description: The major light-harvesting antenna complex II (LHCII) in photosyntheticeukaryotes is located in the thylakoid membrane of the chloroplast. It is aheterotrimeric complex formed by up to 3 different individual subtypes ofchlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb2 is often codedby several nuclear genes and is found together with Lhcb1 within the mobileLHCII trimers involved in state1-state2 transition.

Specificity: Predicted reactivity: angiosperms (monocots and dicots), gymnosperms, mosses, green algae.

Application Details

Application Notes: Recommended Dilution 1 : 5000 with standard ECL (WB). Additional Information: A molecular characterisation of the LHCII proteins can be found in Caffarri et al.(2004) A Look within LHCII: Differential Analysis of the Lhcb1−3 ComplexesBuilding the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis.Biochemistry 43 (29): 9467–9476 .

Buffer: Total IgG in PBS pH 7.4, lyophilized

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Publications

Publications: Andersson, Walters, Horton et al.: "Antisense inhibition of the photosynthetic antenna proteins CP29 and CP26: implications for the mechanism of protective energy dissipation." in: The Plant cell, Vol. 13, Issue 5, pp. 1193-204, 2001 (PubMed).

Asakura, Hirohashi, Kikuchi et al.: "Maize mutants lacking chloroplast FtsY exhibit pleiotropic defects in the biogenesis of thylakoid membranes." in: The Plant cell, Vol. 16, Issue 1, pp. 201-14, 2004 (PubMed).

Tanaka, Tanaka: "Effects of chlorophyllide a oxygenase overexpression on light acclimation in Arabidopsis thaliana." in: Photosynthesis research, Vol. 85, Issue 3, pp. 327-40, 2005 (PubMed).

Tikkanen, Nurmi, Suorsa et al.: "Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants." in: Biochimica et biophysica acta, Vol. 1777, Issue 5, pp. 425-32, 2008 (PubMed).

Tikkanen, Nurmi, Kangasjaervi et al.: "Core protein phosphorylation facilitates the repair of photodamaged photosystem II at high light." in: Biochimica et biophysica acta, 2008 (PubMed).

Yuan, Xu, Yuan et al.: "Light regulation to chlorophyll synthesis and plastid development of the chlorophyll-less golden-leaf privet." in: Journal of integrative plant biology, Vol. 52, Issue 9, pp. 809-16, 2010 (PubMed).