Lhcii Type I Chlorophyll A/B-Binding Protein (Lhcb1) antibody

Antigen: Lhcii Type I Chlorophyll A/B-Binding Protein (Lhcb1)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Arabidopsis thaliana, Peanut (Arachis hypogaea), Barley (Hordeum vulgare), Tomato (Solanum lycopersicum), Mesembryanthemum crystallinum, Tobacco (Nicotiana tabacum), Rice (Oryza sativa), Pea (Pisum sativum), Spinach (Spinacia oleracea), Maize/Corn (Zea mays), Chlamydomonas reinhardtii (C. reinhardtii)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunolocalization (IL)

Catalog no.: ABIN93510

Additional Information

Immunogen: BSA-conjugated synthetic peptide derived from a highly conserved sequence ofLhb1 proteins from angiosperms (monocots and dicots) and gymnosperms,including Arabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2),At1g29930 (Lhcb1.3, most expressed), At2g34430 (Lhcb1.4), and At2g34420(Lhcb1.5)

Format: Lyophilized

Description: The major light-harvesting antenna complex II (LHCII) in photosyntheticeukaryotes is located in the thylakoid membrane of the chloroplast. It is aheterotrimeric complex formed by up to 3 different individual subtypes ofchlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the mostabundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often iscoded by several nuclear genes.

Specificity: Reactivities tested in Western Blotting. Predicted reactivity: angiosperms (monocots and dicots), gymnosperms, mosses, green algae.

Application Details

Application Notes: Recommended Dilution 1 : 2000, detected with standard ECL (WB), 1: 100 - 1: 500 (IG). Additional Information: A molecular characterisation of the LHCII proteins can be found in Caffarri et al.(2004) A Look within LHCII: Differential Analysis of the Lhcb1−3 ComplexesBuilding the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis.Biochemistry 43 (29): 9467–9476 .

Buffer: Total IgG in PBS pH 7.4, lyophilized

Storage: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Research Area: Plant/Algal Physiology

Restrictions: For Research Use only

Publications

Publications: Yoshida, Watanabe, Kato et al.: "Influence of chloroplastic photo-oxidative stress on mitochondrial alternative oxidase capacity and respiratory properties: a case study with Arabidopsis yellow variegated 2." in: Plant & cell physiology, Vol. 49, Issue 4, pp. 592-603, 2008 (PubMed).

Tikkanen, Nurmi, Suorsa et al.: "Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants." in: Biochimica et biophysica acta, Vol. 1777, Issue 5, pp. 425-32, 2008 (PubMed).

Munekage, Eymery, Rumeau et al.: "Elevated expression of PGR5 and NDH-H in bundle sheath chloroplasts in C4 flaveria species." in: Plant & cell physiology, Vol. 51, Issue 4, pp. 664-8, 2010 (PubMed).

Yin, Lundin, Bertrand et al.: "Role of Thylakoid ATP/ADP Carrier in Photoinhibition and Photoprotection of Photosystem II in Arabidopsis." in: Plant physiology, 2010 (PubMed).

Yuan, Xu, Yuan et al.: "Light regulation to chlorophyll synthesis and plastid development of the chlorophyll-less golden-leaf privet." in: Journal of integrative plant biology, Vol. 52, Issue 9, pp. 809-16, 2010 (PubMed).

Saito, Iino, Sonoike et al.: "Remodeling of the major light-harvesting antenna protein of PSII protects the young leaves of barley (Hordeum vulgare L.) from photoinhibition under prolonged iron deficiency." in: Plant & cell physiology, Vol. 51, Issue 12, pp. 2013-30, 2010 (PubMed).

Schmied, Hedtke, Grimm: "Overexpression of HEMA1 encoding glutamyl-tRNA reductase." in: Journal of plant physiology, 2011 (PubMed).