Lhca2 antibody

Catalog No. ABIN5706927

Product Details anti-Lhca2 Antibody

Target: Lhca2 (psi Type II Chloropyll A/B-Binding Protein (Lhca2))

Reactivity: Oryza sativa, Pisum sativum, Arabidopsis thaliana, Barley, Green bean, Moss, Nicotiana tabacum

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Characteristics: Expected / apparent Molecular Weight of the Antigene: 23 / 24 kDa for Arabidopsis thaliana

Purification: affinity purified

Immunogen: BSA-conjugated synthetic peptide derived from the Lhca2 protein of Arabidopsis thaliana UniProt: Q9SYW8, Q8LCQ4, TAIR: At3g61470. This sequence is highly conserved in Lhca2 proteins of angiosperms (monocots and dicots) and gymnosperms as well as in At3g19150. This gene codes for the very low expressed Lhca6 protein which also has been denoted as Lhca2*1.

Isotype: IgG

Application Details

Application Notes: Recommended Dilution: 1 : 2000 - 1 : 5000, detected with standard ECL (WB).

Comment:

A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and16kDa. D1 degradation is a complex set of events and the products observed canbe influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559,shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA freepeptide, indicating that all bands are indeed PsbA and its precursors or breakdown product.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-Lhca2 antibody (ABIN5706927)

Scotti, Sannino, Idoine, Hamman, De Stradis, Giorio, Maréchal-Drouard, Bock, Cardi: "The HIV-1 Pr55(gag) polyprotein binds to plastidial membranes and leads to severe impairment of chloroplast biogenesis and seedling lethality in transplastomic tobacco plants." in: Transgenic research, (2014) (PubMed).

Busch, Petersen, Webber-Birungi, Powikrowska, Lassen, Naumann-Busch, Nielsen, Ye, Boekema, Jensen, Lunde, Jensen: "Composition and structure of photosystem I in the moss Physcomitrella patens." in: Journal of experimental botany, Vol. 64, Issue 10, pp. 2689-99, (2013) (PubMed).

Yamatani, Sato, Masuda, Kato, Morita, Fukunaga, Nagamura, Nishimura, Sakamoto, Tanaka, Kusaba: "NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - protein complexes during leaf senescence." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 4, pp. 652-62, (2013) (PubMed).

Target Details for Lhca2

Target: Lhca2 (psi Type II Chloropyll A/B-Binding Protein (Lhca2))

Background: AGI Code: At3g61470
The light-harvesting protein Lhca2 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca2 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca2 forms a heterodimer (LHCI-680) with Lhca3 that associates with the PSI core close to PsaF and PsaK.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073.

Molecular Weight: expected: 23 kDa, apparent: 24 kDa for Arabidopsis thaliana

UniProt: Q8LCQ4, Q9SYW8