Alpha-Actin (smooth muscle) antibody

Antigen: Alpha-Actin (smooth muscle)

Clonality: Monoclonal (1A4)

Host: Mouse

Reactivity: Human, Vertebrate

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN93631

Additional Information

Immunogen: Synthetic N-terminal decapeptide of smooth muscle Alpha-Actin

Format: Liquid

Isotype: IgG2a

Clone: 1A4

Specificity: Alpha-Actin, smooth muscle

Application Details

Application Notes: C, P, WB , No protease pre-treatment necessary, but microwave treatment usually will intensify staining. We refer to Unmasking Fluid G . Positive Control: Intestine, Appendix

Buffer: stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 ul working solution / test)

Storage: 2-8°C

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Publications

Hasegawa T, Hirose T, Kudo E et al.: "Cytoskeletal characteristics of myofibroblasts in benign neoplastic and reactive fibroblastic lesions." in: Virchows Arch A Pathol Anat Histopathol, Vol. 416, Issue 5, pp. 375-82, 1990 (PubMed).

Ronnov-Jessen L, van Deurs B, Celis JE et al.: "Smooth muscle differentiation in cultured human breast gland stromal cells." in: Lab Invest, Vol. 63, Issue 4, pp. 532-43, 1990 (PubMed).

Skalli, Schuerch, Seemayer et al.: "Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 60, Issue 2, pp. 275-85, 1989 (PubMed).

Skalli, Ropraz, Trzeciak et al.: "A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 2, pp. 2787-96, 1987 (PubMed).