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alpha-Actin (Smooth Muscle) antibody

Details for Product No. ABIN93631, Supplier: Log in to see
Human, Vertebrate
Clonality (Clone)
Monoclonal ()
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Immunogen Synthetic N-terminal decapeptide of smooth muscle Alpha-Actin
Clone 1A4
Isotype IgG2a
Specificity Alpha-Actin, smooth muscle
Research Area Extracellular Matrix
Application Notes C, P, WB , No protease pre-treatment necessary, but microwave treatment usually will intensify staining. We refer to Unmasking Fluid G . Positive Control: Intestine, Appendix
Restrictions For Research Use only
Format Liquid
Buffer stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 ul working solution / test)
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Background publications Ronnov-Jessen L,van Deurs B,Celis JE,Petersen OW: "Smooth muscle differentiation in cultured human breast gland stromal cells." in: Lab Invest, Vol. 63, Issue 4, pp. 532-43, 1990 (PubMed).

Hasegawa T,Hirose T,Kudo E,Abe J,Hizawa K: "Cytoskeletal characteristics of myofibroblasts in benign neoplastic and reactive fibroblastic lesions." in: Virchows Arch A Pathol Anat Histopathol, Vol. 416, Issue 5, pp. 375-82, 1990 (PubMed).

Skalli, Schürch, Seemayer, Lagacé, Montandon, Pittet, Gabbiani: "Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 60, Issue 2, pp. 275-85, 1989 (PubMed).

Skalli, Ropraz, Trzeciak, Benzonana, Gillessen, Gabbiani: "A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 2, pp. 2787-96, 1987 (PubMed).