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Alpha-Actin, (sarcomeric) antibody

Details for Product No. ABIN93634, Supplier: Log in to see
Antigen
Reactivity
Human, Amphibian, Bony fish (Cyprinodontiformes) , Cow (Bovine), Guinea Pig, Reptile, Rabbit, Rat (Rattus), Sheep (Ovine)
2
1
1
1
1
1
1
1
1
Host
Mouse
2
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
2
2
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Immunogen Purified rabbit skeletal muscle actin
Clone 5C5
Isotype IgM
Specificity Alpha-Actin sarcomeric (a-sr-1) specific for skeletal- and heart-muscle
Research Area Extracellular Matrix
Pathways
Application Notes C, P , No protease pre-treatment necessary. Blocking of endogenous peroxidase and alkaline phosphatase recommended depending on the detection system in use. neat Positive Control: Skelet muscle
Restrictions For Research Use only
Format Liquid
Buffer stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 ul working solution / test)
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Background publications Hasegawa T, Matsuno Y, Niki T et al.: "Second primary rhabdomyosarcomas in patients with bilateral retinoblastoma: a clinicopathologic and immunohistochemical study." in: Am J Surg Pathol, Vol. 22, Issue 11, pp. 1351-60, 1998 (PubMed).

Skalli, Gabbiani, Babaui et al.: "Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. II. Rhabdomyosarcomas." in: The American journal of pathology, Vol. 130, Issue 3, pp. 515-31, 1988 (PubMed).

Babai, Skalli, Schurch et al.: "Chemically induced rhabdomyosarcomas in rats. Ultrastructural, immunohistochemical, biochemical features and expression of alpha-actin isoforms." in: Virchows Archiv. B, Cell pathology including molecular pathology, Vol. 55, Issue 5, pp. 263-77, 1988 (PubMed).

Schuerch, Skalli, Seemayer et al.: "Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. I. Smooth muscle tumors." in: The American journal of pathology, Vol. 128, Issue 1, pp. 91-103, 1987 (PubMed).