alpha Sarcomeric Actin antibody

Antigen: alpha Sarcomeric Actin

Clonality: Monoclonal (5C5)

Host: Mouse

Reactivity: Human, Amphibian, Bony fish, Cow (Bovine), Guinea Pig, Reptiles, Rabbit, Rat (Rattus), Sheep (Ovine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN93634

Additional Information

Alternative name: Alpha-Actin, sarcomeric

Immunogen: Purified rabbit skeletal muscle actin

Format: Ready to use

Isotype: IgM  (Matching secondary antibodies)

Clone: 5C5

Description: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 50 immunohistochemical tests (100 ul working solution / test). Use appropriate antibody diluent.

Specificity: Alpha-Actin sarcomeric (a-sr-1) specific for skeletal- and heart-muscle

Application Details

Application Notes: C, P , No protease pre-treatment necessary. Blocking of endogenous peroxidase and alkaline phosphatase recommended depending on the detection system in use. neat Positive Control: Skelet muscle

Buffer: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test)

Storage: 2-8°C

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Publications

Publications: Schuerch, Skalli, Seemayer et al.: "Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. I. Smooth muscle tumors." in: The American journal of pathology, Vol. 128, Issue 1, pp. 91-103, 1987 (PubMed).

Babai, Skalli, Schurch et al.: "Chemically induced rhabdomyosarcomas in rats. Ultrastructural, immunohistochemical, biochemical features and expression of alpha-actin isoforms." in: Virchows Archiv. B, Cell pathology including molecular pathology, Vol. 55, Issue 5, pp. 263-77, 1988 (PubMed).

Skalli, Gabbiani, Babaui et al.: "Intermediate filament proteins and actin isoforms as markers for soft tissue tumor differentiation and origin. II. Rhabdomyosarcomas." in: The American journal of pathology, Vol. 130, Issue 3, pp. 515-31, 1988 (PubMed).

Hasegawa T, Matsuno Y, Niki T et al.: "Second primary rhabdomyosarcomas in patients with bilateral retinoblastoma: a clinicopathologic and immunohistochemical study." in: Am J Surg Pathol, Vol. 22, Issue 11, pp. 1351-60, 1998 (PubMed).