BrdU, Bromodesoxyuridine antibody

Antigen: BrdU, Bromodesoxyuridine

Clonality: Monoclonal (IIB5)

Host: Mouse

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN93648

Additional Information

Immunogen: Bromodesoxyuridine BSA-conjugate

Format: Liquid

Isotype: IgG1  (Matching secondary antibodies)

Clone: IIB5

Description: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 ul working solution / test). Use appropriate antibody diluent.

Specificity: Bromodesoxyuridine and Iododesoxyuridine in denatured single stranded DNA

Application Details

Application Notes: The antibody may be used on paraffin or cryostat material (2,3). Additionally it is suitable for the detection of BrdU by flow cytometry (1). Formaldehyde-fixed paraffin sections are treted with Pepsin and must undergo acid denaturation in front of antibody application. Cryostat material is fixed with alcohol and denatured with acid to generate single stranded DNA.

Buffer: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 ul working solution / test)

Storage: 2-8°C

Research Area: Proliferation Markers

Restrictions: For Research Use only

Publications

Publications: Schutte, Reynders, van Assche et al.: "An improved method for the immunocytochemical detection of bromodeoxyuridine labeled nuclei using flow cytometry." in: Cytometry, Vol. 8, Issue 4, pp. 372-6, 1987 (PubMed).

Schutte, Reynders, Bosman et al.: "Effect of tissue fixation on anti-bromodeoxyuridine immunohistochemistry." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 35, Issue 11, pp. 1343-5, 1987 (PubMed).

Schutte B, Reynders MM, Bosman FT et al.: "Studies with anti-bromodeoxyuridine antibodies: II. Simultaneous immunocytochemical detection of antigen expression and DNA synthesis by in vivo labeling of mouse intestinal mucosa." in: J Histochem Cytochem, Vol. 35, Issue 3, pp. 371-4, 1987 (PubMed).