Cytokeratin (HMW) antibody

Antigen: Cytokeratin (HMW)

Clonality: Monoclonal (34betaE12)

Host: Mouse

Reactivity: Human, Monkey, Rabbit, Horse (Equine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN93714

Additional Information

Immunogen: Cytokeratin extract of human stratum corneum

Format: Liquid

Isotype: IgG1

Clone: 34betaE12

Specificity: High molecular weight cytokeratins (56-57 and 66-68 kDa)

Application Details

Application Notes: Der Antikörper kann auf Paraffin- oder Kryostatmaterial eingesetzt werden. Fixierung mit Methacarn oder Carnoys Fixativ ist der Formaldehydfixierung vorzuziehen. Vorbehandlung: Zur Vorbehandlung von Paraffinschnitten wird Demaskierungslösung C (Art. Nr. D neat

Buffer: stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 ul working solution / test)

Storage: 2-8°C

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Publications

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Gown, Vogel: "Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues." in: The American journal of pathology, Vol. 114, Issue 2, pp. 309-21, 1984 (PubMed).

Yang XJ, Lecksell K, Gaudin P et al.: "Rare expression of high-molecular-weight cytokeratin in adenocarcinoma of the prostate gland: a study of 100 cases of metastatic and locally advanced prostate cancer." in: Am J Surg Pathol, Vol. 23, Issue 2, pp. 147-52, 1999 (PubMed).