Cytokeratin 8/18 antibody

Antigen: Cytokeratin 8/18

Clonality: Monoclonal (5D3)

Host: Mouse

Reactivity: Human, Cat (Feline), Lizard, Sheep (Ovine)

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN93732

Additional Information

Immunogen: Cytokeratins isolated from human mamma carcinoma cell line MCF-7

Format: Liquid

Isotype: IgG2a

Clone: 5D3

Specificity: Human intermediar filament cytokeratins 8 and 18 (MW 52,5 resp. 45 kDa)

Application Details

Application Notes: C, P , In formaldehyde-fixed material pre-treatment with trypsin (0,1% in TBS containing 0,1% CaCl2, pH 7.8) 15 -30 min at 37°C is recommended. 1/20 Positive Control: Skin (sweat glands)

Buffer: stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 ul working solution / test)

Storage: 2-8°C

Restrictions: For Research Use only

Publications

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Gown, Vogel: "Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues." in: The American journal of pathology, Vol. 114, Issue 2, pp. 309-21, 1984 (PubMed).

Yang XJ, Lecksell K, Gaudin P et al.: "Rare expression of high-molecular-weight cytokeratin in adenocarcinoma of the prostate gland: a study of 100 cases of metastatic and locally advanced prostate cancer." in: Am J Surg Pathol, Vol. 23, Issue 2, pp. 147-52, 1999 (PubMed).