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Cytokeratin 8/18 antibody

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Antigen
Reactivity
Human, Cat (Feline), Lizard, Sheep (Ovine)
130
8
7
7
6
4
1
1
Host
Mouse
130
3
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
109
96
91
51
30
19
18
5
5
3
2
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Immunogen Cytokeratins isolated from human mamma carcinoma cell line MCF-7
Clone 5D3
Isotype IgG2a
Specificity Human intermediar filament cytokeratins 8 and 18 (MW 52,5 resp. 45 kDa)
Application Notes C, P , In formaldehyde-fixed material pre-treatment with trypsin (0,1% in TBS containing 0,1% CaCl2, pH 7.8) 15 -30 min at 37°C is recommended. 1/20 Positive Control: Skin (sweat glands)
Restrictions For Research Use only
Format Liquid
Buffer stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 ul working solution / test)
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Background publications Yang, Lecksell, Gaudin, Epstein: "Rare expression of high-molecular-weight cytokeratin in adenocarcinoma of the prostate gland: a study of 100 cases of metastatic and locally advanced prostate cancer." in: The American journal of surgical pathology, Vol. 23, Issue 2, pp. 147-52, 1999 (PubMed).

Gown, Vogel: "Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues." in: The American journal of pathology, Vol. 114, Issue 2, pp. 309-21, 1984 (PubMed).

Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).