Keratin Acidic (AE1) antibody

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Antigen
  • CK1
  • EHK
  • EHK1
  • EPPK
  • K1
  • KRT1A
  • NEPPK
  • keratin 1
  • KRT1
Reactivity
Human, Cow (Bovine), Chicken, Mouse (Murine), Rat (Rattus), Rabbit
48
17
11
11
11
9
9
5
3
3
2
2
Host
Mouse
28
15
5
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
27
19
18
15
13
4
4
2
Options
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Immunogen Human epithelial keratins
Clone AE1
Isotype IgG1
Specificity Acidic cytokeratins (40, 48, 50, 50', 56,5 kDa) according to pI < 5.7
Research Area Extracellular Matrix
Application Notes Working concentration: 1:20 bis 1:50. Positive control: Appendix, epidermis. Improved staining with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000). Incubation time: 60 min at RT. The volume is sufficient for at least 200 immunohistochemical tests (100 ul working solution / test).
Restrictions For Research Use only
Format Liquid
Concentration 50 µg/ml
Buffer stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 ul working solution / test)
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Background publications Yang, Lecksell, Gaudin, Epstein: "Rare expression of high-molecular-weight cytokeratin in adenocarcinoma of the prostate gland: a study of 100 cases of metastatic and locally advanced prostate cancer." in: The American journal of surgical pathology, Vol. 23, Issue 2, pp. 147-52, 1999 (PubMed).

Gown, Vogel: "Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues." in: The American journal of pathology, Vol. 114, Issue 2, pp. 309-21, 1984 (PubMed).

Moll, Franke, Schiller, Geiger, Krepler: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).