Keratin Acidic (AE1) antibody

Antigen: Keratin Acidic (AE1)

Synonyms: K1, CK1, EHK, EHK1, EPPK, KRT1A, NEPPK

Clonality: Monoclonal (AE1)

Host: Mouse

Reactivity: Human, Cow (Bovine), Chicken, Mouse (Murine), Rat (Rattus), Rabbit

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN93735

Additional Information

Alternative name: Cytokeratins, acidic (AE1)

Immunogen: Human epithelial keratins

Format: Liquid

Isotype: IgG1

Clone: AE1

Specificity: Acidic cytokeratins (40, 48, 50, 50', 56,5 kDa) according to pI < 5.7

Application Details

Application Notes: Working concentration: 1:20 bis 1:50. Positive control: Appendix, epidermis. Improved staining with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000). Incubation time: 60 min at RT. The volume is sufficient for at least 200 immunohistochemical tests (100 ul working solution / test).

Concentration: 50 µg/ml

Buffer: stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide. The volume is sufficient for at least 100 immunohistochemical tests (100 ul working solution / test)

Storage: 2-8°C

Research Area: Extracellular Matrix

Restrictions: For Research Use only

Publications

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Gown, Vogel: "Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues." in: The American journal of pathology, Vol. 114, Issue 2, pp. 309-21, 1984 (PubMed).

Yang XJ, Lecksell K, Gaudin P et al.: "Rare expression of high-molecular-weight cytokeratin in adenocarcinoma of the prostate gland: a study of 100 cases of metastatic and locally advanced prostate cancer." in: Am J Surg Pathol, Vol. 23, Issue 2, pp. 147-52, 1999 (PubMed).