HEA125, Epithelium-Specific Glycoprotein, Egp34 antibody

Antigen: HEA125, Epithelium-Specific Glycoprotein, Egp34

Clonality: Monoclonal (HEA125)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN93766

Additional Information

Alternative name: HEA125, Epithelium-specific Glycoprotein, Egp34 (CD326)

Immunogen: Living human colon carcinoma cell line HT-29

Format: Ready to use

Isotype: IgG1  (Matching secondary antibodies)

Clone: HEA125

Description: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide** and BSA. The volume is sufficient for at least 50 immunohistochemical tests (100 ul working solution / test). Use appropriate antibody diluent.

Specificity: 34 kDa Epithelium-specific membrane glycoprotein HEA 125

Application Details

Application Notes: IHC (C,P), FACS , Pre-treatment with pronase 0.1% or unmasking fluid C Positive Control: Appendix

Buffer: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide and BSA. The volume is sufficient for at least 50 immunohistochemical tests (100 µl working solution / test)

Storage: 2-8°C

Research Area: Cell/Tissue Markers

Restrictions: For Research Use only

Publications

Publications: Kemmner W, Moldenhauer G, Schlag P et al.: "Separation of tumor cells from a suspension of dissociated human colorectal carcinoma tissue by means of monoclonal antibody-coated magnetic beads." in: J Immunol Methods, Vol. 147, Issue 2, pp. 197-200, 1992 (PubMed).

Simon, Podolsky, Moldenhauer et al.: "Epithelial glycoprotein is a member of a family of epithelial cell surface antigens homologous to nidogen, a matrix adhesion protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 7, pp. 2755-9, 1990 (PubMed).

Moldenhauer G, Momburg F, Moller P et al.: "Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker." in: Br J Cancer, Vol. 56, Issue 6, pp. 714-21, 1987 (PubMed).

Momburg, Moldenhauer, Haemmerling et al.: "Immunohistochemical study of the expression of a Mr 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues." in: Cancer research, Vol. 47, Issue 11, pp. 2883-91, 1987 (PubMed).