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TUBB3 antibody (Tubulin, Beta, 3) (N-Term)

Details for Product anti-TUBB3 Antibody No. ABIN93911, Supplier: Log in to see
Antigen
  • 3200002H15Rik
  • AI325297
  • beta-4
  • CDCBM
  • CFEOM3A
  • M(beta)3
  • M(beta)4
  • M(beta)6
  • Tubb
  • tubb3
  • TUBB3
  • Tubb4
  • TUBB4
Alternatives
anti-All Species TUBB3 antibody for Western Blotting
Epitope
N-Term
33
29
11
7
5
5
4
4
4
4
3
3
2
2
1
1
Reactivity
All Species
191
57
54
11
5
4
4
2
2
1
1
Host
Mouse
103
89
14
12
1
Clonality (Clone)
Monoclonal ()
Conjugate
This TUBB3 antibody is un-conjugated
24
12
11
3
3
3
3
2
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
180
81
81
44
43
37
33
16
13
8
6
2
2
2
Supplier
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Immunogen Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Clone TU-20
Isotype IgG1
Specificity The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
Purification Purified by ammonium sulphate and caprylic acid precipitation
Purity > 95 % (by SDS-PAGE)
Alternative Name betaIII-tubulin (TUBB3 Antibody Abstract)
Background The betaIII-tubulin isoform is present dominantly in cells of neuronal origin and it is one of the earliest markers of neuronal differentiation. It is regarded as a specific probe for the cells of neuronal origin as well as for the tumours originating from these cells. The betaIII-tubulin is most abundant in cells of neuronal origin, but was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues.
Pathways Microtubule Dynamics
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Immunofluorescence (IF) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (N-Term) (ABIN93911) Immunofluorescence staining (mouse neuroblastoma cells) Immunofluorescence staining o...
Immunofluorescence (IF) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (N-Term) (ABIN93911) Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to...
Immunofluorescence (IF) image for anti-TUBB3 antibody (Tubulin, Beta, 3) (N-Term) (ABIN93911) Immunofluorescence staining (mouse embryonal carcinoma cells) Immunofluorescence stai...
Product cited in: Katsetos, Draber, Kavallaris: "Targeting βIII-tubulin in glioblastoma multiforme: from cell biology and histopathology to cancer therapeutics." in: Anti-cancer agents in medicinal chemistry, Vol. 11, Issue 8, pp. 719-28, 2012 (PubMed).

Gun?ova: "The neurodegenerative process in a neurotoxic rat model and in patients with Huntington's disease: Histopathological parallels and differences." in: Acta histochemica, 2011 (PubMed).

Kunova, Matulka, Eiselleova, Trckova, Hampl, Dvorak: "Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells." in: Reproductive biomedicine online, Vol. 21, Issue 5, pp. 676-86, 2010 (PubMed).

Theodorou, Dalembert, Heffelfinger, White, Weissman, Corcoran, Snyder: "A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation." in: Genes & development, Vol. 23, Issue 5, pp. 575-88, 2009 (PubMed).

Jirásek, Cipro, Musilová, Kubecová, Mandys: "Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody." in: The Indian journal of medical research, Vol. 129, Issue 1, pp. 89-94, 2009 (PubMed).

Dráberová, Del Valle, Gordon, Marková, Smejkalová, Bertrand, de Chadarévian, Agamanolis, Legido, Khalili, Dráber, Katsetos: "Class III beta-tubulin is constitutively coexpressed with glial fibrillary acidic protein and nestin in midgestational human fetal astrocytes: implications for phenotypic identity." in: Journal of neuropathology and experimental neurology, Vol. 67, Issue 4, pp. 341-54, 2008 (PubMed).

Katsetos, Dráberová, Smejkalová, Reddy, Bertrand, de Chadarévian, Legido, Nissanov, Baas, Dráber: "Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells." in: Neurochemical research, Vol. 32, Issue 8, pp. 1387-98, 2007 (PubMed).

Jirásek, Písaríková, Viklický, Mandys: "Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies." in: Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society, Vol. 45, Issue 1, pp. 41-5, 2007 (PubMed).

Kukharskyy, Sulimenko, Mac?rek, Sulimenko, Dráberová, Dráber: "Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells." in: Experimental cell research, Vol. 298, Issue 1, pp. 218-28, 2004 (PubMed).

P?knicová, Kubátová, Sulimenko, Dráberová, Viklický, Hozák, Dráber: "Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail." in: Biology of reproduction, Vol. 65, Issue 3, pp. 672-9, 2001 (PubMed).

Zíková, Sulimenko, Dráber, Dráberová: "Accumulation of 210 kDa microtubule-interacting protein in differentiating P19 embryonal carcinoma cells." in: FEBS letters, Vol. 473, Issue 1, pp. 19-23, 2000 (PubMed).

Dráberová, Lukás, Ivanyi, Viklický, Dráber: "Expression of class III beta-tubulin in normal and neoplastic human tissues." in: Histochemistry and cell biology, Vol. 109, Issue 3, pp. 231-9, 1998 (PubMed).

Background publications Silva, Pereira, Oliveira, Relvas, Rego: "BDNF and extracellular matrix regulate differentiation of mice neurosphere-derived cells into a GABAergic neuronal phenotype." in: Journal of neuroscience research, Vol. 87, Issue 9, pp. 1986-96, 2009 (PubMed).

Woltjen, Michael, Mohseni, Desai, Mileikovsky, Haemaelaeinen, Cowling, Wang, Liu, Gertsenstein, Kaji, Sung, Nagy: "piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells." in: Nature, Vol. 458, Issue 7239, pp. 766-70, 2009 (PubMed).