CD10 antibody (Biotin)

Antigen: CD10

Clonality: Monoclonal (LT10)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Catalog no.: ABIN93935

Additional Information

Gene ID: 3q25.1-q25.2

Immunogen: mouse NALM-6 leukemia pre-B cell line (tissue/cell preparation)

Isotype: IgG1

Clone: LT10

Description: CD10 (neutral endopeptidase NEP, common acute lymphocytic leukemia antigen CALLA,membrane metallo-endopeptidase MME, enkefalinase) is a 100-kDa cell surface zincmetalloprotease cleaving peptide bonds on the N-terminus of hydrophobic amino acids andinactivating multiple physiologically active peptids. CD10 is expressed on various normal celltypes, including lymphoid precursor cells, germinal center B lymhocytes, and some epithelialcells, and its expression level serves as a marker for diagnostics of many carcinomas. CD10is also a differentiation antigen for early B-lymphoid progenitors in the B-cell differentiationpathway and has a key role in regulation of growth, differentiation and signal transduction ofmany cellular systems.

Specificity: The antibody LT10 reacts with CD10 antigen (CALLA - Common acute lymphatic leukemiaantigen), a 100 kDa type II integral membrane protein.

Application Details

Application Notes: Biotinylated antibody is designed for indirect immunofluorescence analysis by FlowCytometry. Suggested working dilution is 1: 200.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Concentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Cancer, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Adaptive Immunity, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Cupedo, Crellin, Papazian et al.: "Human fetal lymphoid tissue-inducer cells are interleukin 17-producing precursors to RORC+ CD127+ natural killer-like cells." in: Nature immunology, Vol. 10, Issue 1, pp. 66-74, 2008 (PubMed).