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Integrin, alpha L (Antigen CD11A (p180), Lymphocyte Function-Associated Antigen 1; alpha Polypeptide) (ITGAL) antibody
|Synonyms||CD11A, LFA-1, LFA1A, Cd11a, Ly-15, Ly-21, ITGAL, CD11a|
Alternatives Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func)
|9 references available|
|Quantity||0.1 mg (1 mg/ml) (Variants)|
|Price||290.40 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 4 to 5 Business Days|
|Immunogen||Leukocytes from a pacient suffering from a LGL-type leukaemia.|
|Description||CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1(LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex.LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation.Cell stimulation by chemokines or other signals leads to induction the high-affinityconformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesionmolecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells andin their tissue-specific settlement, but participates also in control of cell differentiation andproliferation and of T-cell effector functions. Blocking of LFA-1 function by specificantibodies or small molecules has become an important therapeutic approach in treatmentof multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab(Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding ofcholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulationand increase in lymphocytic cholinergic activity.|
|Specificity||The antibody MEM-25 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDatype I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes,macrophages, neutrophils, basophils and eosinophils.HLDA IV, WS Code NL 209|
|Application Notes||Immunoprecipitation: excellent antibody for immunoaffinity purification of LFA-1 complex. Functional Application: The antibody MEM-25 partially blocks binding of LFA-1 complex to ICAM-1.|
|Purity||> 95% (by SDS-PAGE)|
|Purification||Purified from ascites by protein-A affinity chromatography.|
|Buffer||Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4|
|Storage||Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.|
|Research Area||Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
|Restrictions||For Research Use only|
Ottonello, Epstein, Dapino et al.: "Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins." in: Blood, Vol. 93, Issue 10, pp. 3505-11, 1999 (PubMed).
Simon, Cherapanov, Nadra et al.: "Signaling functions of L-selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2891-901, 1999 (PubMed).
Garnotel, Rittiue, Poitevin et al.: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000 (PubMed).
Hajishengallis, Martin, Sojar et al.: "Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 403-11, 2002 (PubMed).
Mathison, Befus, Davison et al.: "Modulation of neutrophil function by the tripeptide feG." in: BMC immunology, Vol. 4, pp. 3, 2003 (PubMed).
Stulc, Vrabluik, Kasalovua et al.: "Leukocyte and endothelial adhesion molecules in patients with hypercholesterolemia: the effect of atorvastatin treatment." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 57, Issue 2, pp. 184-94, 2008 (PubMed).
Wang, Kwas, Wu: "Intercellular adhesion molecule 1 (ICAM-1), but not ICAM-2 and -3, is important for dendritic cell-mediated human immunodeficiency virus type 1 transmission." in: Journal of virology, Vol. 83, Issue 9, pp. 4195-204, 2009 (PubMed).
Eksteen, Mora, Haughton et al.: "Gut homing receptors on CD8 T cells are retinoic acid dependent and not maintained by liver dendritic or stellate cells." in: Gastroenterology, Vol. 137, Issue 1, pp. 320-9, 2009 (PubMed).
Aubert, Yoon, Sloan et al.: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).