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ITGAL antibody (Integrin alpha-L) (alpha subunit)

Details for Product anti-ITGAL Antibody No. ABIN93948, Supplier: Log in to see
Antigen
  • (p180)
  • Cd11a
  • LFA-1
  • LFA-1A
  • Ly-15
  • Ly-21
  • CD11A
  • LFA1A
  • CD11a
Epitope
alpha subunit
19
18
11
3
3
2
2
2
1
1
1
1
Reactivity
Human
325
204
36
15
11
10
10
8
4
3
Host
Mouse
344
176
58
16
Clonality (Clone)
Monoclonal ()
Conjugate
This ITGAL antibody is un-conjugated
95
80
48
17
13
6
6
5
4
3
3
3
2
2
2
2
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
473
166
99
98
79
68
47
27
16
12
8
6
6
4
3
2
2
2
1
1
1
1
Supplier
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Immunogen Leukocytes from a pacient suffering from a LGL-type leukaemia.
Clone MEM-25
Isotype IgG1
Specificity The antibody MEM-25 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. HLDA IV, WS Code NL 209
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name ITGAL (ITGAL Antibody Abstract)
Background CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Research Area Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Application Notes Flow Cytometry: Recommended dilution: 2 µg/mL
Immunoprecipitation: excellent antibody for immunoaffinity purification of LFA-1 complex
Functional Application: The antibody MEM-25 partially blocks binding of LFA-1 complex to ICAM-1.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-ITGAL antibody (Integrin alpha-L) (alpha subunit) (ABIN93948) Surface staining of human peripheral blood cells with anti-human CD11a (MEM-25) PE.
Product cited in: Aubert, Yoon, Sloan et al.: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).

Wang, Kwas, Wu: "Intercellular adhesion molecule 1 (ICAM-1), but not ICAM-2 and -3, is important for dendritic cell-mediated human immunodeficiency virus type 1 transmission." in: Journal of virology, Vol. 83, Issue 9, pp. 4195-204, 2009 (PubMed).

Ottonello, Epstein, Mancini et al.: "Monoclonal LYM-1 antibody-dependent cytolysis by human neutrophils exposed to GM-CSF: auto-regulation of target cell attack by cathepsin G." in: Journal of leukocyte biology, Vol. 75, Issue 1, pp. 99-105, 2004 (PubMed).

Mathison, Befus, Davison et al.: "Modulation of neutrophil function by the tripeptide feG." in: BMC immunology, Vol. 4, pp. 3, 2003 (PubMed).

Hajishengallis, Martin, Sojar et al.: "Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 403-11, 2002 (PubMed).

Garnotel, Rittié, Poitevin et al.: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000 (PubMed).

Simon, Cherapanov, Nadra et al.: "Signaling functions of L-selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2891-901, 1999 (PubMed).

Background publications Eksteen, Mora, Haughton et al.: "Gut homing receptors on CD8 T cells are retinoic acid dependent and not maintained by liver dendritic or stellate cells." in: Gastroenterology, Vol. 137, Issue 1, pp. 320-9, 2009 (PubMed).

Stulc, Vrabluik, Kasalovua et al.: "Leukocyte and endothelial adhesion molecules in patients with hypercholesterolemia: the effect of atorvastatin treatment." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 57, Issue 2, pp. 184-94, 2008 (PubMed).

Fujii, Takada-Takatori, Kawashima: "Roles played by lymphocyte function-associated antigen-1 in the regulation of lymphocytic cholinergic activity." in: Life sciences, Vol. 80, Issue 24-25, pp. 2320-4, 2007 (PubMed).

Kellersch, Kolanus: "Membrane-proximal signaling events in beta-2 integrin activation." in: Results and problems in cell differentiation, Vol. 43, pp. 245-57, 2006 (PubMed).

Giblin, Lemieux: "LFA-1 as a key regulator of immune function: approaches toward the development of LFA-1-based therapeutics." in: Current pharmaceutical design, Vol. 12, Issue 22, pp. 2771-95, 2006 (PubMed).

Ottonello, Epstein, Dapino et al.: "Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins." in: Blood, Vol. 93, Issue 10, pp. 3505-11, 1999 (PubMed).