ITGAL antibody (Integrin alpha-L) (alpha subunit)

Details for Product anti-ITGAL Antibody No. ABIN93948, Supplier: Log in to see
Antigen
  • CD11A
  • LFA-1
  • LFA1A
  • (p180)
  • Cd11a
  • LFA-1A
  • Ly-15
  • Ly-21
  • CD11a
  • ITGAL
  • integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
  • integrin alpha L
  • ITGAL
  • Itgal
Alternatives
anti-Human ITGAL antibody for Functional Studies
Epitope
alpha subunit
17
16
12
11
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
513
225
47
34
22
19
14
11
11
10
8
7
6
4
3
2
1
1
Host
Mouse
531
195
80
19
1
1
Clonality (Clone)
Monoclonal ()
Conjugate
This ITGAL antibody is un-conjugated
97
86
60
20
19
17
17
15
15
14
13
12
11
11
11
11
10
9
9
8
5
4
4
4
3
3
2
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
700
209
185
165
117
113
65
50
32
19
17
14
12
11
5
4
3
3
2
2
1
1
1
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Leukocytes from a pacient suffering from a LGL-type leukaemia.
Clone MEM-25
Isotype IgG1
Specificity The antibody MEM-25 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
HLDA IV, WS Code NL 209
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name ITGAL (ITGAL Antibody Abstract)
Background CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
Research Area Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways Activated T Cell Proliferation, Integrin Complex
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-Integrin alpha-L (ITGAL) (alpha subunit) antibody (ABIN93948) Surface staining of human peripheral blood cells with anti-human CD11a (MEM-25) PE.
Product cited in: Aubert, Yoon, Sloan, Spear, Jerome: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).

Wang, Kwas, Wu: "Intercellular adhesion molecule 1 (ICAM-1), but not ICAM-2 and -3, is important for dendritic cell-mediated human immunodeficiency virus type 1 transmission." in: Journal of virology, Vol. 83, Issue 9, pp. 4195-204, 2009 (PubMed).

Ottonello, Epstein, Mancini, Dapino, Dallegri: "Monoclonal LYM-1 antibody-dependent cytolysis by human neutrophils exposed to GM-CSF: auto-regulation of target cell attack by cathepsin G." in: Journal of leukocyte biology, Vol. 75, Issue 1, pp. 99-105, 2004 (PubMed).

Mathison, Befus, Davison, Woodman: "Modulation of neutrophil function by the tripeptide feG." in: BMC immunology, Vol. 4, pp. 3, 2003 (PubMed).

Hajishengallis, Martin, Sojar, Sharma, Schifferle, DeNardin, Russell, Genco: "Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 403-11, 2002 (PubMed).

Garnotel, Rittié, Poitevin, Monboisse, Nguyen, Potron, Maquart, Randoux, Gillery: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000 (PubMed).

Simon, Cherapanov, Nadra, Waddell, Seo, Wang, Doerschuk, Downey: "Signaling functions of L-selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2891-901, 1999 (PubMed).

Background publications Eksteen, Mora, Haughton, Henderson, Lee-Turner, Villablanca, Curbishley, Aspinall, von Andrian, Adams: "Gut homing receptors on CD8 T cells are retinoic acid dependent and not maintained by liver dendritic or stellate cells." in: Gastroenterology, Vol. 137, Issue 1, pp. 320-9, 2009 (PubMed).

Stulc, Vrabluik, Kasalovua, Marinov, Svobodovua, Ceska: "Leukocyte and endothelial adhesion molecules in patients with hypercholesterolemia: the effect of atorvastatin treatment." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 57, Issue 2, pp. 184-94, 2008 (PubMed).

Fujii, Takada-Takatori, Kawashima: "Roles played by lymphocyte function-associated antigen-1 in the regulation of lymphocytic cholinergic activity." in: Life sciences, Vol. 80, Issue 24-25, pp. 2320-4, 2007 (PubMed).

Kellersch, Kolanus: "Membrane-proximal signaling events in beta-2 integrin activation." in: Results and problems in cell differentiation, Vol. 43, pp. 245-57, 2006 (PubMed).

Giblin, Lemieux: "LFA-1 as a key regulator of immune function: approaches toward the development of LFA-1-based therapeutics." in: Current pharmaceutical design, Vol. 12, Issue 22, pp. 2771-95, 2006 (PubMed).

Ottonello, Epstein, Dapino, Barbera, Morone, Dallegri et al.: "Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b ..." in: Blood, Vol. 93, Issue 10, pp. 3505-11, 1999 (PubMed).

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