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Integrin, alpha L (Antigen CD11A (p180), Lymphocyte Function-Associated Antigen 1; alpha Polypeptide) (ITGAL) antibody (FITC)
|Synonyms||CD11A, LFA-1, LFA1A, Cd11a, Ly-15, Ly-21, ITGAL, CD11a|
Alternatives Flow Cytometry (FACS)
|10 references available|
|Quantity||100 tests (Variants)|
|Price||363.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 4 to 5 Business Days|
|Immunogen||Leukocytes from a pacient suffering from a LGL-type leukaemia.|
|Description||CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1(LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex.LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation.Cell stimulation by chemokines or other signals leads to induction the high-affinityconformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesionmolecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells andin their tissue-specific settlement, but participates also in control of cell differentiation andproliferation and of T-cell effector functions. Blocking of LFA-1 function by specificantibodies or small molecules has become an important therapeutic approach in treatmentof multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab(Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding ofcholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulationand increase in lymphocytic cholinergic activity.|
|Specificity||The antibody MEM-25 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDatype I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes,macrophages, neutrophils, basophils and eosinophils.HLDA IV, WS Code NL 209|
|Application Notes||The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.|
|Buffer||The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.|
|Storage||Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.|
|Research Area||Stem Cells, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
|Restrictions||For Research Use only|
Ottonello, Epstein, Dapino et al.: "Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins." in: Blood, Vol. 93, Issue 10, pp. 3505-11, 1999 (PubMed).
Simon, Cherapanov, Nadra et al.: "Signaling functions of L-selectin in neutrophils: alterations in the cytoskeleton and colocalization with CD18." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2891-901, 1999 (PubMed).
Garnotel, Rittiue, Poitevin et al.: "Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 11, pp. 5928-34, 2000 (PubMed).
Hajishengallis, Martin, Sojar et al.: "Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction." in: Clinical and diagnostic laboratory immunology, Vol. 9, Issue 2, pp. 403-11, 2002 (PubMed).
Mathison, Befus, Davison et al.: "Modulation of neutrophil function by the tripeptide feG." in: BMC immunology, Vol. 4, pp. 3, 2003 (PubMed).
Stulc, Vrabluik, Kasalovua et al.: "Leukocyte and endothelial adhesion molecules in patients with hypercholesterolemia: the effect of atorvastatin treatment." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 57, Issue 2, pp. 184-94, 2008 (PubMed).
Wang, Kwas, Wu: "Intercellular adhesion molecule 1 (ICAM-1), but not ICAM-2 and -3, is important for dendritic cell-mediated human immunodeficiency virus type 1 transmission." in: Journal of virology, Vol. 83, Issue 9, pp. 4195-204, 2009 (PubMed).
Eksteen, Mora, Haughton et al.: "Gut homing receptors on CD8 T cells are retinoic acid dependent and not maintained by liver dendritic or stellate cells." in: Gastroenterology, Vol. 137, Issue 1, pp. 320-9, 2009 (PubMed).
Aubert, Yoon, Sloan et al.: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, 2009 (PubMed).
Cancer: Lesport, Baudhuin, Sousa et al.: "Inhibition of human Vγ9Vδ2 T-cell antitumoral activity through HLA-G: implications for immunotherapy of cancer." in: Cellular and molecular life sciences : CMLS, 2011 (PubMed).
(Sample species: Human).