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CD19 antibody (CD19 Molecule) (Biotin)

Details for Product anti-CD19 Antibody No. ABIN94014, Supplier: Log in to see
Antigen
  • CD19
  • AW495831
  • B4
  • CVID3
Alternatives
anti-Human CD19 antibody for Immunoprecipitation
Reactivity
Human
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
This CD19 antibody is conjugated to Biotin
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Application
Flow Cytometry (FACS)
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Supplier
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Immunogen Daudi human Burkitt lymphoma cell line
Clone LT19
Isotype IgG1
Specificity The antibody LT19 reacts with CD19 (B4), a 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells, it is lost on plasma cells.
Characteristics The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Purification Purified from ascites by precipitation methods and size-exclusion chromatography.
Alternative Name CD19 (CD19 Antibody Abstract)
Background CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.
Research Area Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Biotinylated antibody is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working dilution is 1: 200. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD19 antibody (CD19 Molecule)  (Biotin) (ABIN94014) Surface staining of human peripheral blood cells with anti-human CD19 (LT19) APC.
Product cited in: Kayserova, Vcelakova, Stechova et al.: "Decreased dendritic cell numbers but increased TLR9-mediated interferon-alpha production in first degree relatives of type 1 diabetes patients." in: Clinical immunology (Orlando, Fla.), Vol. 153, Issue 1, pp. 49-55, 2014 (PubMed).

Elias, Flo, Lopez et al.: "Strong cytosine-guanosine-independent immunostimulation in humans and other primates by synthetic oligodeoxynucleotides with PyNTTTTGT motifs." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 7, pp. 3697-704, 2003 (PubMed).

Background publications Rayment, Kooij, Zhang et al.: "Evidence for the specificity for platelet HPA-1a alloepitope and the presenting HLA-DR52a of diverse antigen-specific helper T cell clones from alloimmunized mothers." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 1, pp. 677-86, 2009 (PubMed).

Gonçalves, Castro, Henriques et al.: "Molecular cloning and analysis of SSc5D, a new member of the scavenger receptor cysteine-rich superfamily." in: Molecular immunology, Vol. 46, Issue 13, pp. 2585-96, 2009 (PubMed).

Svensson: "Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology." in: Methods in molecular biology (Clifton, N.J.), Vol. 531, pp. 187-202, 2009 (PubMed).

Harrison, Franklin, Campbell: "Enumeration of blood dendritic cells in patients with multiple myeloma at presentation and through therapy." in: Leukemia & lymphoma, Vol. 49, Issue 12, pp. 2272-83, 2008 (PubMed).

Allen, Pang, Skowera et al.: "Plasmacytoid dendritic cells are proportionally expanded at diagnosis of type 1 diabetes and enhance islet autoantigen presentation to T-cells through immune complex capture." in: Diabetes, Vol. 58, Issue 1, pp. 138-45, 2008 (PubMed).

Barat, Gilbert, Imbeault et al.: "Extracellular ATP reduces HIV-1 transfer from immature dendritic cells to CD4+ T lymphocytes." in: Retrovirology, Vol. 5, pp. 30, 2008 (PubMed).

Shao, Suresh, Vakil et al.: "Pivotal Advance: Th-1 cytokines inhibit, and Th-2 cytokines promote fibrocyte differentiation." in: Journal of leukocyte biology, Vol. 83, Issue 6, pp. 1323-33, 2008 (PubMed).

Shi, Xie, Chang et al.: "CD19 hyperexpression augments Sle1-induced humoral autoimmunity but not clinical nephritis." in: Arthritis and rheumatism, Vol. 56, Issue 9, pp. 3057-69, 2007 (PubMed).

Vantourout, Martinez, Fabre et al.: "Ecto-F1-ATPase and MHC-class I close association on cell membranes." in: Molecular immunology, Vol. 45, Issue 2, pp. 485-92, 2007 (PubMed).

Inabe, Kurosaki: "Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement." in: Blood, Vol. 99, Issue 2, pp. 584-9, 2002 (PubMed).

Fujimoto, Poe, Jansen et al.: "CD19 amplifies B lymphocyte signal transduction by regulating Src-family protein tyrosine kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7088-94, 1999 (PubMed).