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CD25 antibody

IL2RA Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-140 unconjugated
Catalog No. ABIN94049
  • Target See all CD25 (IL2RA) Antibodies
    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
    Reactivity
    • 170
    • 107
    • 35
    • 9
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 128
    • 80
    • 78
    • 2
    • 1
    Mouse
    Clonality
    • 208
    • 77
    • 2
    Monoclonal
    Conjugate
    • 105
    • 31
    • 26
    • 25
    • 19
    • 9
    • 6
    • 5
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    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD25 antibody is un-conjugated
    Application
    • 201
    • 85
    • 50
    • 47
    • 39
    • 32
    • 19
    • 17
    • 14
    • 14
    • 8
    • 6
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    • 1
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    Flow Cytometry (FACS), Immunoprecipitation (IP)
    Specificity
    The antibody MEM-140 reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells), it is lost on resting B and T lymphocytes.
    Cross-Reactivity (Details)
    Human
    Purification
    Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    PHA-activated peripheral blood leucocytes
    Clone
    MEM-140
    Isotype
    IgM
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    Discover our top product IL2RA Primary Antibody
  • Application Notes
    Immunoprecipitation: Excellent for immunoisolation of CD25+ cells.
    Flow cytometry: Recommended dilution: 1 μg/mL.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Target
    CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))
    Alternative Name
    CD25 (IL2RA Products)
    Synonyms
    CD25 antibody, IDDM10 antibody, IL2R antibody, TCGFR antibody, IL2RAC antibody, IL-2RA antibody, Il2r antibody, Ly-43 antibody, interleukin 2 receptor subunit alpha antibody, interleukin-2 receptor subunit alpha-like antibody, interleukin 2 receptor, alpha chain antibody, IL2RA antibody, Il2ra antibody, LOC100222957 antibody
    Background
    Interleukin 2 receptor subunit alpha,CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 Molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.,IL-2R α, chain, IL-2-RA, IL2-RA, p55, TAC antigen, IDDM10, TCGFR
    Gene ID
    3559
    UniProt
    P01589
    Pathways
    JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
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