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CD3 antibody (CD3) (Dimer gamma-epsilon)

Details for Product anti-CD3 Antibody No. ABIN94058, Supplier: Log in to see
Antigen
  • CD3
  • CD3d molecule, delta (CD3-TCR complex)
  • CD3e molecule, epsilon (CD3-TCR complex)
  • DNA segment, Chr 9, MRC UK Mouse Genome Centre 45 expressed
  • CD3D
  • CD3E
  • D9Mgc45e
Epitope
Dimer gamma-epsilon, Dimer delta-epsilon
12
11
9
9
5
4
3
1
1
1
1
1
Reactivity
Human
797
304
84
43
33
31
30
17
14
13
11
10
10
8
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
3
1
Host
Mouse
886
195
62
47
22
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD3 antibody is un-conjugated
162
119
93
65
30
25
24
22
21
21
17
17
14
13
11
10
9
9
8
8
8
8
8
8
6
6
4
4
4
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
973
254
214
146
120
117
106
101
76
56
19
16
12
11
11
10
10
7
6
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
Options
Supplier
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Immunogen Human thymocytes and T lymphocytes.
Clone MEM-57
Isotype IgG2a
Specificity The antibody MEM-57 reacts with gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.
HLDA IV., WS Code T 96
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name CD3 (CD3 Antibody Abstract)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD3 antibody (CD3) (Dimer gamma-epsilon) (ABIN94058) Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC. C...
Product cited in: Majer, Vlaskova, Krol, Kalina, Kubanek, Stolnaya, Dvorakova, Elleder, Sikora: "Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency." in: Gene, Vol. 498, Issue 2, pp. 183-95, 2012 (PubMed).

Mookerjee-Basu, Vantourout, Martinez, Perret, Collet, Puerigaud, Peyrottes, Champagne: "F1-Adenosine Triphosphatase Displays Properties Characteristic of an Antigen Presentation Molecule for V{gamma}9V{delta}2 T Cells." in: Journal of immunology (Baltimore, Md. : 1950), 2010 (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Panyi, Bagdány, Bodnár, Vámosi, Szentesi, Jenei, Mátyus, Varga, Waldmann, Gáspar, Damjanovich: "Colocalization and nonrandom distribution of Kv1.3 potassium channels and CD3 molecules in the plasma membrane of human T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2592-7, 2003 (PubMed).

Dave, Cao, Browne, Alarcon, Fernandez-Miguel, Lafaille, de la Hera, Tonegawa, Kappes: "CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage." in: The EMBO journal, Vol. 16, Issue 6, pp. 1360-70, 1997 (PubMed).

Soucek, Hilgert, Budová, Lindnerová: "Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin-2." in: Neoplasma, Vol. 41, Issue 2, pp. 75-81, 1994 (PubMed).

Hilgert, Fran?k, Stefanová, Kaslík, Jirka, Kristofová, Rossmann, Soucek, Horejsi: "Therapeutic in vivo use of the A1-CD3 monoclonal antibody." in: Transplantation, Vol. 55, Issue 2, pp. 435-8, 1993 (PubMed).

Soucek, Chudomel, Hrubá, Lindnerová: "Induction of NK and LAK activities in human lymphocyte culture by a cytosol fraction from leukemic myeloblasts and by monoclonal antibody CD 3." in: Neoplasma, Vol. 38, Issue 1, pp. 33-41, 1991 (PubMed).

Background publications Svensson: "Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology." in: Methods in molecular biology (Clifton, N.J.), Vol. 531, pp. 187-202, 2009 (PubMed).

Jursik, Prchal, Grillari-Voglauer, Drbal, Fuertbauer, Jungfer, Albert, Steinhuber, Hemetsberger, Grillari, Stockinger, Katinger: "Large-scale production and characterization of novel CD4+ cytotoxic T cells with broad tumor specificity for immunotherapy." in: Molecular cancer research : MCR, Vol. 7, Issue 3, pp. 339-53, 2009 (PubMed).

Muhammad, Schiller, Forster, Eckerstorfer, Geyeregger, Leksa, Zlabinger, Sibilia, Sonnleitner, Paster, Stockinger: "Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 12, pp. 7672-80, 2009 (PubMed).

Paster, Paar, Eckerstorfer, Jakober, Drbal, Schütz, Sonnleitner, Stockinger: "Genetically encoded Förster resonance energy transfer sensors for the conformation of the Src family kinase Lck." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 4, pp. 2160-7, 2009 (PubMed).

Todaro, DAsaro, Caccamo, Iovino, Francipane, Meraviglia, Orlando, La Mendola, Gulotta, Salerno, Dieli, Stassi: "Efficient killing of human colon cancer stem cells by gammadelta T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 11, pp. 7287-96, 2009 (PubMed).

Houben, Voigt, Noelke, Hofmeister, Becker, Schrama: "MAPK-independent impairment of T-cell responses by the multikinase inhibitor sorafenib." in: Molecular cancer therapeutics, Vol. 8, Issue 2, pp. 433-40, 2009 (PubMed).

Kuhns, Davis, Garcia: "Deconstructing the form and function of the TCR/CD3 complex." in: Immunity, Vol. 24, Issue 2, pp. 133-9, 2006 (PubMed).

Alarcón, Swamy, van Santen, Schamel: "T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity." in: EMBO reports, Vol. 7, Issue 5, pp. 490-5, 2006 (PubMed).

Huang, Wange: "T cell receptor signaling: beyond complex complexes." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28827-30, 2004 (PubMed).

Horejsí, Angelisová, Bazil, Kristofová, Stoyanov, Stefanová, Hausner, Vosecký, Hilgert et al.: "Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) ..." in: Folia biologica, Vol. 34, Issue 1, pp. 23-34, 1988 (PubMed).