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CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (Chain epsilon)

Details for Product anti-CD3E Antibody No. ABIN94065, Supplier: Log in to see
Antigen
  • AI504783
  • CD3
  • cd3e
  • CD3E
  • CD3epsilon
  • T3E
  • t3e
  • T3e
  • TCRE
  • tcre
Alternatives
anti-Human CD3E antibody for Flow Cytometry
Epitope
Chain epsilon
30
23
19
15
15
14
14
12
8
8
6
6
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
480
420
108
74
48
28
27
26
26
26
21
13
9
8
7
7
6
5
5
2
2
2
2
1
1
Host
Mouse
383
213
211
74
19
13
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD3E antibody is un-conjugated
95
81
66
39
21
20
19
19
17
17
15
15
15
12
12
12
12
12
12
11
10
10
10
7
6
6
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
684
279
210
166
110
91
86
83
75
38
24
10
9
9
6
4
3
3
2
2
1
1
1
1
1
1
1
Supplier
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Immunogen Human peripheral blood lymphocytes.
Clone MEM-92
Isotype IgM
Specificity The antibody MEM-92 reacts with epsilon chain of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.
HLDA IV., WS Code T 97
Purification Purified from ascites by thiophilic adsorption-affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name CD3E (CD3E Antibody Abstract)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T-cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells
Pathways TCR Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Meraner, Horejsí, Wolpl, Fischer, Stingl, Maurer: "Dendritic cells sensitize TCRs through self-MHC-mediated Src family kinase activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 4, pp. 2262-71, 2007 (PubMed).

Batista, Millán, Mittelbrunn, Sánchez-Madrid, Alonso: "Recruitment of transferrin receptor to immunological synapse in response to TCR engagement." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 172, Issue 11, pp. 6709-14, 2004 (PubMed).

Brdicková, Brdicka, Angelisová, Horváth, Spicka, Hilgert, Paces, Simeoni, Kliche, Merten, Schraven, Horejsí: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, 2002 (PubMed).

Stulnig, Berger, Sigmund, Stockinger, Horejsí, Waldhäusl: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, 1997 (PubMed).

Soucek, Hilgert, Budová, Lindnerová: "Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin-2." in: Neoplasma, Vol. 41, Issue 2, pp. 75-81, 1994 (PubMed).

Background publications Alarcón, Swamy, van Santen, Schamel: "T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity." in: EMBO reports, Vol. 7, Issue 5, pp. 490-5, 2006 (PubMed).

Kuhns, Davis, Garcia: "Deconstructing the form and function of the TCR/CD3 complex." in: Immunity, Vol. 24, Issue 2, pp. 133-9, 2006 (PubMed).

Huang, Wange: "T cell receptor signaling: beyond complex complexes." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28827-30, 2004 (PubMed).

Stefanová, Saville, Peters, Cleghorn, Schwartz, Venzon, Weinhold, Jack, Bartholomew, Blattner, Yarchoan, Bolen, Horak: "HIV infection--induced posttranslational modification of T cell signaling molecules associated with disease progression." in: The Journal of clinical investigation, Vol. 98, Issue 6, pp. 1290-7, 1996 (PubMed).