CD4 Molecule (CD4) antibody (Dyomics 647)

Antigen: CD4 Molecule (CD4)

Synonyms: CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25

Clonality: Monoclonal (MEM-241)

Host: Mouse

Reactivity: Human

Conjugate: Dyomics 647

Application: Flow Cytometry (FACS)

Catalog no.: ABIN94093

Additional Information

Alternative name: CD4

Gene ID: 12pter-p12

Immunogen: 2 N-terminal domains of human CD4 fused to human IgG1 Fc

Isotype: IgG1

Clone: MEM-241

Description: CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulinsupergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-likeV-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consistsof 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain ofCD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4segregates into specific detergent-resistant T-cell membrane microdomains.Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1),HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds toCD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1),L-selectinIntracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHCclass II molecules) and HIV infection (human immunodeficiency virus, CD4 is primaryreceptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-celladhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigenpresentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost completeabsence in patients blood, tissue and organs (SCID immunodeficiency).

Specificity: The antibody MEM-241 recognizes CD4 antigen, a 55 kDa transmebrane glycoproteinexpressed on a subset of T lymphocytes (helper T-cells) and also on monocytes, tissuemacrophages and granulocytes.HCDM (former HLDA VIII) Meeting, May 2006, Québec, Canada, WS Code M241

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Reactivity of the antibody MEM-241 with human CD4 immunoprecipitated from T cells by the antibody MEM-115. T cells isolated from fresh buffy coats were solubilized in lauryl maltoside lysis buffer and the lysate was subjected to affinity chromatography both on an immunosorbent made of immobilized antibody MEM-115 (CD4) or irrelevant negative control immunosorbent. Western blot was stained with the antibody MEM-241. Lanes: 1. original lysate, 2. the material eluted from irrelevant immunosorbent (negative control), 3. the material eluted from MEM-115 (CD4)-Sepharose.

Publications

Publications: Manasa, Musabaike, Masimirembwa et al.: "Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe." in: Clinical and vaccine immunology : CVI, Vol. 14, Issue 3, pp. 293-8, 2007 (PubMed).

Anderson, Sayers, Haniffa et al.: "Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells." in: Journal of leukocyte biology, Vol. 84, Issue 1, pp. 124-33, 2008 (PubMed).

Karlsson, Cowley, Martinez et al.: "Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3." in: Experimental hematology, Vol. 36, Issue 9, pp. 1167-75, 2008 (PubMed).